Effects of Gender and Estrogen Receptors on Iron-Induced Brain Edema Formation.

Abstract

Our previous studies have shown that female mice have less brain edema and better recovery in neurological deficits after intracerebral hemorrhage (ICH) and that 17β-estradiol treatment in male mice markedly reduces ICH-induced brain edema. In this study, we investigated the role of gender and the estrogen receptors (ERs) in iron-induced brain edema. There were three parts in this study: (1) either male or female mice received an injection of 10 μL FeCl2 (1 mM) into the right caudate; (2) females received an intracaudate injection of FeCl2 or saline with 1 μg of ICI 182,780 (antagonists of ERs) or vehicle; and (3) males were treated with the ER regulator tamoxifen (5 mg/kg subcutaneously) or vehicle 1 h after FeCl2 injection. Mice were euthanized 24 h later for brain edema determination. FeCl2 induced lower brain edema in females than in males. Co-injection of ICI 182,780 with FeCl2 aggravated iron-induced brain edema in female mice. ICI 182,780 itself did not induce brain edema at the dose of 1 μg. Tamoxifen treatment reduced FeCl2-induced brain edema in male mice. In conclusion, iron induced less brain edema in female mice than in males. ER modification can affect iron-induced brain edema.

• Authors: Qing Xie, Guohua Xi, Richard F Keep, Ya Hua
• Journal: Acta neurochirurgica. Supplement (Acta Neurochir Suppl) Vol. 121 Pg. 341-5 ( 2016) ISSN: 0065-1419 [Print] Austria
• PMID: 26463972 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Chlorides
• Estrogen Antagonists
• Estrogen Receptor Antagonists
• Iron Compounds
• Receptors, Estrogen
• Tamoxifen
• Fulvestrant
• Estradiol
• Iron

Topics

• Animals
• Brain (drug effects, metabolism)
• Brain Edema (chemically induced, metabolism)
• Caudate Nucleus
• Chlorides (toxicity)
• Disease Models, Animal
• Estradiol (analogs & derivatives, pharmacology)
• Estrogen Antagonists (pharmacology)
• Estrogen Receptor Antagonists (pharmacology)
• Female
• Fulvestrant
• Iron
• Iron Compounds (toxicity)
• Male
• Mice
• Mice, Inbred C57BL
• Receptors, Estrogen (antagonists & inhibitors, metabolism)
• Sex Factors
• Tamoxifen (pharmacology)