Analysis of Altered Micro RNA Expression Profiles in Focal Cortical Dysplasia IIB.

Abstract

Focal cortical dysplasia type IIB is a commonly encountered subtype of developmental malformation of the cerebral cortex and is often associated with pharmacoresistant epilepsy. In this study, to investigate the molecular etiology of focal cortical dysplasia type IIB, the authors performed micro ribonucleic acid (RNA) microarray on surgical specimens from 5 children (2 female and 3 male, mean age was 73.4 months, range 50-112 months) diagnosed of focal cortical dysplasia type IIB and matched normal tissue adjacent to the lesion. In all, 24 micro RNAs were differentially expressed in focal cortical dysplasia type IIB, and the microarray results were validated using quantitative real-time polymerase chain reaction (PCR). Then the putative target genes of the differentially expressed micro RNAs were identified by bioinformatics analysis. Moreover, biological significance of the target genes was evaluated by investigating the pathways in which the genes were enriched, and the Hippo signaling pathway was proposed to be highly related with the pathogenesis of focal cortical dysplasia type IIB.

• Authors: Lin Li, Chang-Qing Liu, Tian-Fu Li, Yu-Guang Guan, Jian Zhou, Xue-Ling Qi, Yu-Tao Yang, Jia-Hui Deng, Zhi-Qing David Xu, Guo-Ming Luan
• Journal: Journal of child neurology (J Child Neurol) Vol. 31 Issue 5 Pg. 613-20 (Apr 2016) ISSN: 1708-8283 [Electronic] United States
• PMID: 26442942 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © The Author(s) 2015.

Chemical References

• MicroRNAs

Topics

• Biological Ontologies
• Brain (diagnostic imaging)
• Child
• Child, Preschool
• Computational Biology
• Epilepsy (drug therapy, genetics, metabolism)
• Female
• Gene Expression Profiling
• Gene Expression Regulation, Developmental (genetics)
• Hippocampus (metabolism)
• Humans
• Image Processing, Computer-Assisted
• Magnetic Resonance Imaging
• Male
• Malformations of Cortical Development, Group I (drug therapy, genetics, metabolism)
• MicroRNAs (genetics, metabolism)
• Oligonucleotide Array Sequence Analysis