Abstract | BACKGROUND: METHODS: Quantitative real-time reverse transcriptase polymerase chain reaction was employed to assess RNA expression of the target genes in 24 sporadic insulinomas: 15 grade 1 (G1), six grade 2 (G2) and three hepatic metastases. Somatic mutations of MET gene were searched by direct sequencing of exons 2, 10, 14, 16, 17 and 19. RESULTS: Overexpression of MET was observed in the three hepatic metastases concomitantly with upregulation of the genes encoding HGF and matriptase and downregulation of SPINT1. A positive correlation was observed between MET RNA expression and Ki-67 proliferation index while a negative correlation was detected between SPINT1 expression and the mitotic index. No somatic mutations were found in MET gene. CONCLUSION: The final effect of the increased expression of HGF, its activator ( matriptase) and its specific receptor (MET) together with a decreased expression of one potent inhibitor of matriptase (SPINT1) is probably a contribution to tumoral progression and metastatization in insulinomas.
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Authors | Cahuê De Bernardis Murat, Paula Waki Lopes da Rosa, Maria Angela Henriques Zanella Fortes, Luciana Corrêa, Marcel Cerqueira Cesar Machado, Estela Maria Novak, Sheila Aparecida Coelho Siqueira, Maria Adelaide Albergaria Pereira, Maria Lucia Corrêa-Giannella, Daniel Giannella-Neto, Ricardo Rodrigues Giorgi |
Journal | Diabetology & metabolic syndrome
(Diabetol Metab Syndr)
Vol. 7
Pg. 84
( 2015)
ISSN: 1758-5996 [Print] England |
PMID | 26435753
(Publication Type: Journal Article)
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