(177)Lu-Labeled Cerasomes Encapsulating Indocyanine Green for Cancer Theranostics.

Abstract

This Article reported the fabrication of a robust theranostic cerasome encapsulating indocyanine green (ICG) by incorporating 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[carboxy(polyethylene glycol)2000]-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid monoamide (DSPE-PEG2000-DOTA), followed by chelating radioisotope of (177)Lu. Its applications in optical and nuclear imaging of tumor uptake and biodistribution, as well as photothermal killing of cancer cells, were investigated. It was found that the obtained cerasome could act efficiently as fluorescence contrast agent as well as nuclear imaging tracer. Encapsulating ICG into cerasome could protect ICG from degradation, aggregation, and fast elimination from body, resulting in remarkable improvement in near-infrared fluorescence imaging, photothermal stability, and in vivo pharmacokinetic profile. Both fluorescence and nuclear imaging showed that such agent could selectively accumulate in tumor site after intravenous injection of the cerasome agent into Lewis lung carcinoma tumor bearing mice, resulting in efficient photothermal ablation of tumor through a one-time NIR laser irradiation at the best time window. The ability to track the uptake of cerasomes on a whole body basis could provide researchers with an excellent tool for developing cerasome-based drug delivery agents, especially the strategy of labeling cerasomes with theranostic radionuclide (177)Lu, enabling the ability of the (177)Lu-labeled cerasomes for radionuclide cancer therapy and even the combined therapy.

• Authors: Lijia Jing, Jiyun Shi, Di Fan, Yaqian Li, Renfa Liu, Zhifei Dai, Fan Wang, Jie Tian
• Journal: ACS applied materials & interfaces (ACS Appl Mater Interfaces) Vol. 7 Issue 39 Pg. 22095-105 (Oct 07 2015) ISSN: 1944-8252 [Electronic] United States
• PMID: 26398723 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Liposomes
• Radiopharmaceuticals
• Lutetium
• Indocyanine Green

Topics

• Animals
• Antineoplastic Agents (chemistry, pharmacokinetics, pharmacology)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Drug Stability
• Indocyanine Green (chemistry)
• Liposomes (chemistry, pharmacokinetics, pharmacology)
• Lutetium (chemistry)
• Mice
• Phototherapy (methods)
• Radiopharmaceuticals (chemistry)
• Theranostic Nanomedicine (methods)
• Tomography, Emission-Computed, Single-Photon
• Tomography, X-Ray Computed