Cytotoxicity of chitosans with different acetylation degrees and molecular weights on bladder carcinoma cells.

Abstract

The purpose of this research was to evaluate the cytotoxicity of chitosans with different degrees of acetylation (DA) and molecular weights (MW), as well as the effect of their positive ionic charges controlled by pH on bladder carcinoma cells (RT112 and RT112cp) using the tetrazolium salt colorimetric (MTT) assay. Our data showed that all chitosan samples were cytotoxic on RT112 and RT112cp cells with a higher cytotoxicity obtained at lower pH. Further, it was found that the toxicity increased with increasing DA. However, no significant difference in cytotoxicity between chitosans with different molecular weights was observed. Annexin V-FITC staining test was then used to study and quantify the induction of apoptosis. Data shows that chitosans induce apoptosis of RT112 and RT112cp cells with the same dependence with DA.

• Authors: Islem Younes, Véronique Frachet, Marguerite Rinaudo, Kemel Jellouli, Moncef Nasri
• Journal: International journal of biological macromolecules (Int J Biol Macromol) Vol. 84 Pg. 200-7 (Mar 2016) ISSN: 1879-0003 [Electronic] Netherlands
• PMID: 26397996 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015. Published by Elsevier B.V.

Chemical References

• Annexins
• Antineoplastic Agents
• Chitosan
• Cisplatin

Topics

• Acetylation
• Annexins (metabolism)
• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Chitosan (chemistry, pharmacology)
• Cisplatin (pharmacology)
• Drug Resistance, Neoplasm
• Humans
• Hydrogen-Ion Concentration
• Molecular Weight
• Urinary Bladder Neoplasms