S100A12 concentrations and myeloperoxidase activity in the intestinal mucosa of healthy dogs.

Abstract

BACKGROUND:

Relatively few laboratory markers have been evaluated for the detection or monitoring of intestinal inflammation in canine chronic enteropathies, including inflammatory bowel disease (IBD). Previous research found that the intestinal mucosal levels of S100A12 and myeloperoxidase (MPO), as biomarkers of gut inflammation, were elevated in human patients with IBD. To date, the S100A12 and MPO levels in intestinal mucosal samples from either healthy dogs or from dogs suffering from IBD remain unreported. Therefore, this study aimed to evaluate the mucosal S100A12 and MPO levels in four different parts of the intestine (duodenum, jejunum, ileum and colon) in 12 healthy laboratory Beagle dogs using the ELISA and spectrophotometric methods, respectively.

RESULTS:

Based on histological examinations, the recorded findings for all the samples were considered normal. The mucosal concentration of S100A12 in the ileum was significantly higher than in all other segments of the intestine (p < 0.05). MPO activity was significantly higher in the ileal, jejunal and duodenal than in colonic mucosal samples (p < 0.05). Moreover, its concentration was higher in the jejunum than in the duodenum.

CONCLUSIONS:

This study showed that S100A12 and MPO are reliably detectable in canine intestinal mucosa. The assays used appeared to be sufficient to further evaluate the role of S100A12 and MPO in the pathogenesis of canine chronic enteropathies, including IBD. These biomarkers may play a role in the initial detection of gut inflammation suggesting the need for further investigations to confirm IBD or to differentiate between IBD subtypes. Understanding the role of S100A12 and MPO in the pathogenesis of chronic intestinal inflammation in future may result in an improved understanding of canine chronic intestinal inflammation.

• Authors: Mohsen Hanifeh, Romy M Heilmann, Satu Sankari, Minna M Rajamäki, Laura Mäkitalo, Pernilla Syrjä, Susanne Kilpinen, Jan S Suchodolski, Jörg M Steiner, Thomas Spillmann
• Journal: BMC veterinary research (BMC Vet Res) Vol. 11 Pg. 234 (Sep 14 2015) ISSN: 1746-6148 [Electronic] England
• PMID: 26370713 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• S100A12 Protein
• Peroxidase

Topics

• Animals
• Colon (enzymology, metabolism)
• Dogs (metabolism)
• Gene Expression Regulation, Enzymologic (physiology)
• Intestinal Mucosa (enzymology, metabolism)
• Intestine, Small (enzymology, metabolism)
• Peroxidase (genetics, metabolism)
• S100A12 Protein (genetics, metabolism)