Ceramide and N,N,N-Trimethylphytosphingosine-Iodide (TMP-I)-Based Lipid Nanoparticles for Cancer Therapy.

Abstract

PURPOSE:

To evaluate the anti-tumor effect of ceramide or trimethylphytosphingosine-iodide (TMP-I) containing solid lipid nanoparticles (SLNs) prepared using trymyristin, phosphatidylcholine (PC), and Pluronic P85 (P85) for intravenous delivery of docetaxel.

METHODS:

Docetaxel-loaded SLNs using ceramide or TMP-I at 3.22% (w/w) with a mean diameter of 89-137 nm were successfully prepared by high pressure homogenization. The prepared nanoparticles were characterized by particle size, zeta potential, drug content, and TEM analysis. Cellular uptake and cytotoxicity were studied using adriamycin-resistant breast cancer (MCF-7/ADR) cells. The optimized formulation's dissolution profile, pharmacokinetics, and antitumor effect in mice tumor model were compared with that of control (Taxotere(®)).

RESULTS:

The drug release rate of docetaxel from SLNs was lower than that of control (Taxotere(®)). The prepared SLNs showed higher cellular uptake of docetaxel compared to that of Taxotere(®) in MCF-7/ADR cell lines, which was further confirmed by the confocal laser scanning microscopy (CLSM) study using coumarin 6 (C6). Prepared SLNs exhibited significantly increased antitumor efficacy, compared to Taxotere(®), in MCF-7/ADR cells. In vivo pharmacokinetic study in rats (at 10 mg/kg dose) showed that the SLNs significantly reduced in vivo clearance of drug than Taxotere(®). Interestingly, ceramide and TMP-I SLNs efficiently inhibited the tumor growth compared to Taxotere(®) in MCF-7/ADR tumor xenografted mouse model.

CONCLUSION:

This work showed that TMP-I and ceramide SLNs not only significantly enhanced systemic exposure of drug, but also increased antitumor efficacy compared to Taxotere(®) and control SLN.

• Authors: Prabagar Balakrishnan, Chung Kil Song, Alexander Jahn, Hyun-Jong Cho
• Journal: Pharmaceutical research (Pharm Res) Vol. 33 Issue 1 Pg. 206-16 (Jan 2016) ISSN: 1573-904X [Electronic] United States
• PMID: 26337769 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Antineoplastic Agents, Phytogenic
• Ceramides
• Delayed-Action Preparations
• Excipients
• N,N,N-trimethylphytosphingosine
• Phosphatidylcholines
• Quaternary Ammonium Compounds
• Taxoids
• pluronic block copolymer p85
• Docetaxel
• Poloxalene
• Sphingosine

Topics

• Animals
• Antineoplastic Agents (administration & dosage, pharmacokinetics, therapeutic use)
• Antineoplastic Agents, Phytogenic (administration & dosage, chemistry, therapeutic use)
• Cell Line, Tumor
• Ceramides (chemistry)
• Chemistry, Pharmaceutical
• Delayed-Action Preparations
• Docetaxel
• Excipients
• Male
• Mice
• Nanoparticles (chemistry)
• Neoplasms (drug therapy)
• Particle Size
• Phosphatidylcholines (chemistry)
• Poloxalene
• Quaternary Ammonium Compounds (chemistry)
• Rats
• Rats, Sprague-Dawley
• Sphingosine (analogs & derivatives, chemistry)
• Taxoids (administration & dosage, chemistry, therapeutic use)