Protective Effects of Modeled Superoxide Dismutase Coordination Compound (MSODa) Against Ischemia/Reperfusion Injury in Rat Skeletal Muscle.

Abstract

BACKGROUND/AIM:

Ischemia/reperfusion (I/R) injury of skeletal muscles is common pathophysiology during surgeries and the superoxide dismutase (SOD) plays a critical role in this process. SOD-modeled coordination compound (MSODa) may simulate the protective effects as SOD.

METHODS:

Therefore, this study was designed to explore the protective effects and underlying mechanism of MSODa on malondialdehyde (MDA) and integrin-β2 (CD11b/CD18) in plasma, myeloperoxidase (MPO) and intercellular cell adhesion molecule-1 (ICAM-1) in tissue, and morphological changes before and after I/R injury. The rat model of I/R in hind limb was established and randomly divided into sham, ischemia, I/R, I/R-treated with saline, SOD, and MSODa, respectively.

RESULTS:

These results showed that averaged values for MDA, MPO, CD11b/CD18, and ICAM-1 were significantly increased (P < 0.01 vs ischemia alone) in a time-dependent fashion along with marked tissue remodeling, such as abnormal arrangement of muscular fibers, interstitial edema, vasodilation with no-reflow, inflammatory cells adherent and infiltration, structural changes in mitochondrial, and decrease in glycogens as well. However, all parameter changes induced by I/R injury were reversed, at least partially, by MSODa and SOD treatments and intriguingly, the beneficial/protective effects of MSODa was superior to SOD with an early onset.

CONCLUSION:

This novel finding demonstrates that MSODa improves I/R injury of skeletal muscles due at least partially to inhibition of adherent molecule expression and reduction of oxygen free radical formation during I/R pathophysiological processes and this protective action of MSODa was superior to SOD, highlighting the bright future for MSODa in clinical management of tissue I/R injury.

• Authors: Xin-Tao Wang, Ye Tian, Wen-Xiao Xu, Li-Huang Cui, Shou-Yang Xiang, Song-Cen Lü
• Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (Cell Physiol Biochem) Vol. 37 Issue 2 Pg. 465-76 ( 2015) ISSN: 1421-9778 [Electronic] Germany
• PMID: 26315464 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 The Author(s) Published by S. Karger AG, Basel.

Chemical References

• CD18 Antigens
• Intercellular Adhesion Molecule-1
• Malondialdehyde
• Peroxidase
• Superoxide Dismutase

Topics

• Animals
• Biomimetic Materials (administration & dosage, pharmacology)
• CD18 Antigens (blood)
• Disease Models, Animal
• Gene Expression Regulation (drug effects)
• Intercellular Adhesion Molecule-1 (metabolism)
• Male
• Malondialdehyde (blood)
• Muscle, Skeletal (drug effects, injuries, metabolism)
• Peroxidase (metabolism)
• Rats
• Rats, Wistar
• Reperfusion Injury (blood, metabolism, prevention & control)
• Superoxide Dismutase (administration & dosage, chemistry, pharmacology)