Lepidotol A from Mesua lepidota Inhibits Inflammatory and Immune Mediators in Human Endothelial Cells.

Abstract

Phytochemical investigation on the fruits of Mesua lepidota (Calophyllaceae) led to the isolation of seven new phenylcoumarin derivatives named lepidotols A-E (1-5) and lepidotins A and B (6, 7). These structures were elucidated by spectroscopic and spectrometric methods including UV, NMR, and HRMS. Lepidotol A (1), the major compound, was evaluated for its inhibitory effect on inflammation and immunity using endothelial cell-based cellular assays. At 10 μM, 1 exhibited an anti-inflammatory activity, with a significant inhibition of vascular cell adhesion molecule 1 and intercellular adhesion molecule 1 expression induced by tumor necrosis factor-α. Lepidotol A also showed a mild immunosuppressive effect, with inhibition of the major histocompatibility complex molecules, namely, human leukocyte antigen (HLA)-DR and HLA-E.

• Authors: Caroline Rouger, Séverine Derbré, Béatrice Charreau, Angélique Pabois, Thomas Cauchy, Marc Litaudon, Khalijah Awang, Pascal Richomme
• Journal: Journal of natural products (J Nat Prod) Vol. 78 Issue 9 Pg. 2187-97 (Sep 25 2015) ISSN: 1520-6025 [Electronic] United States
• PMID: 26301802 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Coumarins
• Immunologic Factors
• NF-kappa B
• TNF protein, human
• Tumor Necrosis Factor-alpha
• Vascular Cell Adhesion Molecule-1
• lepidotol A
• Intercellular Adhesion Molecule-1

Topics

• Anti-Inflammatory Agents (pharmacology)
• Cell Adhesion (drug effects)
• Coumarins (chemistry, isolation & purification, pharmacology)
• Endothelial Cells (drug effects, metabolism)
• Fruit (chemistry)
• Humans
• Immunologic Factors (pharmacology)
• Inflammation (metabolism)
• Intercellular Adhesion Molecule-1 (metabolism)
• Malpighiaceae (chemistry)
• Molecular Structure
• NF-kappa B (metabolism)
• Tumor Necrosis Factor-alpha
• Vascular Cell Adhesion Molecule-1