MicroRNA-454 functions as an oncogene by regulating PTEN in uveal melanoma.

Abstract

MicroRNAs (miRNAs) regulate gene expression by targeted repression of transcription and translation, and are involved in carcinogenesis. In this study, we demonstrated that the expression of miR-454 was up-regulated in uveal melanoma tissues compared to normal tissues. Ectopic expression of miR-454 resulted in significant promotion of cell proliferation, colony formation, invasion and induction of cell cycle in uveal melanoma cells. Furthermore, we identified PTEN as a direct target of miR-454. Our data revealed that ectopic expression of PTEN restored the effects of miR-454 on cell proliferation and invasion in uveal melanoma cells. These findings support an oncogene role of miR-454 in development of uveal melanoma.

• Authors: Lei Sun, Qiaoling Wang, Xiangchun Gao, Dejing Shi, Shuyong Mi, Qing Han
• Journal: FEBS letters (FEBS Lett) Vol. 589 Issue 19 Pt B Pg. 2791-6 (Sep 14 2015) ISSN: 1873-3468 [Electronic] England
• PMID: 26296312 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Retracted Publication)
• Copyright: Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Chemical References

• MIRN454 microRNA, human
• MicroRNAs
• Proto-Oncogene Proteins c-akt
• TOR Serine-Threonine Kinases
• PTEN Phosphohydrolase
• PTEN protein, human

Topics

• Base Sequence
• Cell Cycle (genetics)
• Cell Line, Tumor
• Cell Proliferation
• Humans
• Melanoma (genetics, metabolism, pathology)
• MicroRNAs (genetics)
• Neoplasm Invasiveness
• Oncogenes
• PTEN Phosphohydrolase (genetics, metabolism)
• Proto-Oncogene Proteins c-akt (metabolism)
• TOR Serine-Threonine Kinases (metabolism)
• Up-Regulation (genetics)
• Uveal Neoplasms (genetics, metabolism, pathology)