Abstract | BACKGROUND: OBJECTIVE: The purpose of the study was to identify correlations between changes in hematologic variables against markers of renal function, blood pressure, and erythropoietin (EPO) in a naturally occurring hypertensive model of CKD, the Lewis polycystic kidney (LPK) rat. METHODS: Complete blood count, systolic blood pressure, urea and creatinine concentration, urinary protein to creatinine ratio, and plasma EPO concentration were determined in control Lewis (n = 51) and LPK rats (n = 56) aged 6-24 weeks. Renal EPO gene expression and RBC osmotic fragility were also documented. Hematopoiesis in spleen and bone marrow were assessed. RESULTS: Lewis polycystic kidney rats had increasing urea and creatinine concentrations, concurrent with the development of a nonregenerative normocytic/normochromic anemia and hypertension, with a significant negative correlation between both HGB and HCT with urea concentration and blood pressure (P < .01). HCT was also significantly negatively correlated with creatinine concentration (P = .014). WBC was significantly negatively correlated with urea (P < .01). Plasma EPO concentration was increased and renal EPO mRNA expression was significantly upregulated in LPK animals. The former was significantly positively correlated with blood pressure and platelet count (P < .05). RBC osmotic fragility was normal in LPK rats and there was no evidence for increased RBC elimination or extramedullary hematopoiesis. CONCLUSIONS: Marked anemia in the LPK CKD rodent model in the presence of elevated EPO suggests inefficient erythropoiesis that is correlated with plasma urea concentration and blood pressure.
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Authors | Jacqueline K Phillips, Rochelle Boyd, Mark B Krockenberger, Gaetan Burgio |
Journal | Veterinary clinical pathology
(Vet Clin Pathol)
Vol. 44
Issue 3
Pg. 342-54
(Sep 2015)
ISSN: 1939-165X [Electronic] United States |
PMID | 26292198
(Publication Type: Journal Article)
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Copyright | © 2015 American Society for Veterinary Clinical Pathology. |
Chemical References |
- Erythropoietin
- Creatinine
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Topics |
- Anemia
(complications, physiopathology)
- Animals
- Blood Cell Count
- Blood Pressure
- Blood Urea Nitrogen
- Cohort Studies
- Creatinine
(blood)
- Disease Models, Animal
- Disease Progression
- Erythropoietin
(blood)
- Female
- Hypertension
(complications, physiopathology)
- Kidney
(physiopathology)
- Kidney Function Tests
- Male
- Polycystic Kidney Diseases
(complications, physiopathology)
- Rats
- Rats, Inbred Lew
- Renal Insufficiency, Chronic
(complications, physiopathology)
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