Immunoprophylactic potential of wheat grass extract on benzene-induced leukemia: An in vivo study on murine model.

Abstract

OBJECTIVES:

Wheat grass (Triticum aestivum) is a gift of nature given to mankind. A number of scientific research on wheatgrass establishes its anticancer and antioxidant potential. Current work was focused to determine antileukemic effect of wheat grass.

MATERIALS AND METHODS:

The commercial wheatgrass powder was extracted with 95% of methanol. Methanol extract of wheat grass was studied for acute oral toxicity as per revised Organization for Economic Cooperation and Development Guidelines number 423. Leukemia was successfully induced in Wister rats by intravenous injection of benzene. The blood was collected and analyzed for hematological parameters. Phagocytotic activity of the extract was determined.

RESULTS:

Phytochemical screening revealed the presence of flavonoids, phenolics, carbohydrates, and amino acids. From acute toxicity studies, it was found that the methanol extract of wheatgrass was safe up to a dose level of 2000 mg/kg of body weight. Outcomes of hematological parameters in various experimental groups of murine model demonstrated antileukemic effect of extract. Methanol extract of wheatgrass aroused the process of phagocytosis of killed Candida albicans and also demonstrated a significant chemotactic activity at all tested concentrations.

CONCLUSION:

In the current work, methanol extract of wheat grass demonstrated antileukemic potential that might be due to the presence of flavonoids and polyphenolics in it. Further isolation, structural characterization of active constituents is necessary to extrapolate the mechanism of action.

• Authors: Neelofar Khan, Aditya Ganeshpurkar, Nazneen Dubey, Divya Bansal
• Journal: Indian journal of pharmacology (Indian J Pharmacol) 2015 Jul-Aug Vol. 47 Issue 4 Pg. 394-7 ISSN: 1998-3751 [Electronic] India
• PMID: 26288471 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents, Phytogenic
• Plant Extracts
• Benzene

Topics

• Animals
• Antineoplastic Agents, Phytogenic (administration & dosage, pharmacology)
• Benzene (toxicity)
• Female
• Leukemia (prevention & control)
• Male
• Plant Extracts (administration & dosage, pharmacology)
• Radiation Dose Hypofractionation
• Rats
• Rats, Wistar
• Toxicity Tests, Acute
• Triticum (chemistry)