UBE2S is associated with malignant characteristics of breast cancer cells.

Abstract	Ubiquitination is essential for various biological processes, such as signal transduction, intracellular trafficking, and protein degradation. Accumulating evidence has demonstrated that ubiquitination plays a crucial role in cancer development. In this report, we examine the expression and function of ubiquitin-conjugating enzyme E2S (UBE2S) in breast cancer. Immunohistochemical analysis revealed that UBE2S is highly expressed in breast cancer. The depletion of UBE2S by siRNA induced disruption of the actin cytoskeleton and focal adhesions. Interestingly, phosphorylation of FAK at Tyr397, which is important for the transduction of integrin-mediated signaling, was significantly reduced by UBE2S knockdown. We also show that UBE2S knockdown suppressed the malignant characteristics of breast cancer cells, such as migration, invasion, and anchorage-independent growth. Our results indicate that UBE2S could be a potential target for breast cancer treatment.
Authors	Akter Khondker Ayesha, Toshinori Hyodo, Eri Asano, Naoki Sato, Mohammed A Mansour, Satoko Ito, Michinari Hamaguchi, Takeshi Senga
Journal	Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (Tumour Biol) Vol. 37 Issue 1 Pg. 763-72 (Jan 2016) ISSN: 1423-0380 [Electronic] Netherlands
PMID	26245992 (Publication Type: Journal Article)
Chemical References	Integrins RNA, Small Interfering Ube2S protein, human Ubiquitin-Conjugating Enzymes Focal Adhesion Protein-Tyrosine Kinases
Topics	Actin Cytoskeleton (metabolism) Aged Anoikis Breast Neoplasms (metabolism) Cell Adhesion Cell Line, Tumor Cell Movement Cell Transformation, Neoplastic Cytoplasm (metabolism) Female Focal Adhesion Protein-Tyrosine Kinases (metabolism) Gene Expression Regulation, Neoplastic Humans Immunohistochemistry Integrins (metabolism) MCF-7 Cells Middle Aged Neoplasm Invasiveness Phosphorylation RNA, Small Interfering (metabolism) Signal Transduction Ubiquitin-Conjugating Enzymes (metabolism) Ubiquitination

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