Melanomas cause over 76% of
skin cancer deaths annually.
Phosphatidylinositol 3-kinase (PI3K)-AKT-mammalian target of
rapamycin (mTOR) signaling pathway is important for
melanoma initiation and progression. In the current study, we evaluated the potential anti-
melanoma effect of
VS-5584, a novel and highly potent PI3K-mTOR dual inhibitor. We demonstrated that
VS-5584 potently inhibited survival and proliferation of established (A375, A-2058 and SK-MEL-3 lines) and primary human
melanoma cells, but was non-cytotoxic to non-cancerous human skin keratinocytes and B10BR murine melanocytes. At the meantime,
VS-5584 induced
caspase-dependent apoptotic death in
melanoma cells, and its cytotoxicity was alleviated by the
caspase inhibitors. At the molecular level,
VS-5584 blocked AKT-mTOR activation and downregulated
cyclin D1 expression in
melanoma cells, while the expressions of Bcl-xL and Bcl-2 were not affected by
VS-5584 treatment. On the other hand, a
BH-3 mimetic Bcl-xL/Bcl-2 inhibitor
ABT-737, as well as
siRNA-mediated knockdown of Bcl-xL or Bcl-2, enhanced the activity of
VS-5584 in
melanoma cells. In vivo,
oral administration of
VS-5584 suppressed A375
melanoma xenograft growth in nude mice, and its activity was further enhanced by co-administration of
ABT-737. These results provide the rationale for the clinical assessment of
VS-5584 in
melanoma patients and development of
ABT-737 and other Bcl-xL/Bcl-2 inhibitors as the possible adjuvants.