Abstract | PURPOSE: METHOD: We used the comparative proteomic approach of two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to compare the proteomic profiles of the same donor liver at three different time points: T1, immediately after cardiac arrest of donors (normal control); T2, before portal vein anastomosis ( ischemia); and T3, 2 h after hepatic artery anastomosis (reperfusion). RESULT: CONCLUSION:
Ischemia and reperfusion during LT may lead to different modifications of the liver proteins. Most metabolic enzymes and antioxidant enzymes were upregulated during ischemia, indicating that lipid metabolic disorder and oxidative stress are closely related to the development of ischemic injury. ER chaperones may play a vital role in mediating I/RI and preventing ER stress caused by I/RI. Modulation of ER chaperones could be used as a key therapeutic target to improve the outcomes of LT.
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Authors | Bin Wu, HongLi Wu, JianNing Chen, XueFeng Hua, Ning Li, MinQiang Lu |
Journal | Hepatology international
(Hepatol Int)
Vol. 7
Issue 1
Pg. 286-98
(Mar 2013)
ISSN: 1936-0533 [Print] United States |
PMID | 26201644
(Publication Type: Journal Article)
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