Huaier aqueous extract inhibits cervical cancer cell proliferation via JNK/p38 pathway.

Abstract	Although the anticancer effects of Huaier extract have been widely investigated, including anti-proliferate, anti-angiogenic and anti-metastatic activities, the mechanisms are not well understood. This study aimed to elucidate the inhibitory effect of Huaier extract on tumor growth in cervical cancer cells and its molecular mechanisms. Cell viability and motility were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, colony assays, migration, and invasive assays, respectively. The distribution of the cell cycle was analyzed by ﬂow cytometry. Huaier inhibited cell viability of SiHa and C33A cells in a time- and dose-dependent manner; cell migration and invasiveness were also suppressed; Huaier was able to cause G2/M cell cycle arrest in C33A cells. The western blot results confirmed Huaier dose-dependently increased expression of phosphorylated c-Jun N-terminal kinase (JNK), p-38 and downregulated the expression of phosphorylated extracellular signal-regulated kinase (ERK) in a time- and dose-dependently manner. In vivo experiments showed that Huaier significantly suppressed the tumor volume of SiHa cell xenografts. These data suggest that Huaier may inhibit tumor proliferation in cervical cancer via the JNK/p38 signaling pathway.
Authors	Li Yan, Xiaolin Liu, Aijun Yin, Yuyan Wei, Qifeng Yang, Beihua Kong
Journal	International journal of oncology (Int J Oncol) Vol. 47 Issue 3 Pg. 1054-60 (Sep 2015) ISSN: 1791-2423 [Electronic] Greece
PMID	26201539 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antineoplastic Agents Plant Extracts
Topics	Animals Antineoplastic Agents (administration & dosage, pharmacology) Cell Line, Tumor Cell Movement (drug effects) Cell Proliferation (drug effects) Cell Survival (drug effects) Dose-Response Relationship, Drug Female Gene Expression Regulation, Neoplastic (drug effects) Humans MAP Kinase Signaling System (drug effects) Mice Plant Extracts (administration & dosage, pharmacology) Uterine Cervical Neoplasms (drug therapy, metabolism) Xenograft Model Antitumor Assays

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