Biologically synthesised silver nanoparticles from three diverse family of plant extracts and their anticancer activity against epidermoid A431 carcinoma.

Abstract

HYPOTHESIS:

Biological synthesis of silver nanoparticles is a cost effective natural process where the phytochemicals specifically phenols, flavonoids and terpenoids present in the plant extracts act as capping and reducing agent. Due to their nano size regime the silver nanoparticles may directly bind to the DNA of the pathogenic bacterial strains leading to higher antimicrobial activity.

EXPERIMENT:

In the current study silver nanoparticles were synthesised using plant extracts from different origin Cucurbita maxima (petals), Moringa oleifera (leaves) and Acorus calamus (rhizome). The synthesised nanoparticles were characterized by UV-visible spectroscopy, dynamic light scattering (DLS), X-ray diffraction (XRD), field emission scanning electron microscopy (Fe-SEM) and Fourier transform infrared spectroscopy (FTIR).

FINDING:

Highly crystalline, roughly spherical and cuboidal silver nanoparticles of 30-70 nm in size were synthesised. The nanoparticles provided strong antimicrobial activity against pathogenic strains. The effect of the synthesised nanoparticles against A431 skin cancer cell line was tested for their toxicity by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye. The IC50 values of 82.39±3.1, 83.57±3.9 and 78.58±2.7 μg/ml were calculated for silver nanoparticles synthesised by C. maxima, M. oleifera and A. calamus respectively.

• Authors: Debasis Nayak, Sonali Pradhan, Sarbani Ashe, Pradipta Ranjan Rauta, Bismita Nayak
• Journal: Journal of colloid and interface science (J Colloid Interface Sci) Vol. 457 Pg. 329-38 (Nov 01 2015) ISSN: 1095-7103 [Electronic] United States
• PMID: 26196716 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• Anti-Bacterial Agents
• Antineoplastic Agents, Phytogenic
• Plant Extracts
• Silver

Topics

• Acorus (chemistry)
• Anti-Bacterial Agents (biosynthesis, chemistry, isolation & purification, pharmacology)
• Antineoplastic Agents, Phytogenic (biosynthesis, chemistry, isolation & purification, pharmacology)
• Bacillus subtilis (drug effects)
• Carcinoma, Squamous Cell (drug therapy, pathology)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Cucurbita (chemistry)
• Dose-Response Relationship, Drug
• Drug Screening Assays, Antitumor
• Escherichia coli (drug effects)
• Humans
• Metal Nanoparticles (chemistry)
• Microbial Sensitivity Tests
• Moringa oleifera (chemistry)
• Particle Size
• Plant Extracts (chemistry)
• Silver (chemistry, metabolism, pharmacology)
• Structure-Activity Relationship
• Surface Properties
• Vibrio cholerae (drug effects)