Augmented miR-10b expression associated with depressed expression of its target gene KLF4 involved in gastric carcinoma.

Abstract

Previous studies have revealed several targets of miR-10b, such as syndecan-1, HOXD10, TBX5, and E-cadherin. In this study, we aimed to assess whether Krüppel-like factor 4 (KLF4) is a target gene of miR-10b in gastric cancer (GC). Targeting of KLF4 by miR-10b was confirmed by dual-luciferase reporter assays. The expression levels of miR-10b and KLF4 mRNA in 5 different gastric cancer cell lines and 65 pairs of gastric cancer tissues were detected by Real-time PCR. In addition, KLF4 protein in gastric cancer cell lines and 30 GC tissues was measured by western blotting and immunochemistry, respectively. KLF4 is a direct target gene of miR-10b in GC, and its expression is reduced by miR-10b at both mRNA and protein levels. In addition, the expression level of miR-10b was tendentiously upregulated in GC tissues while the expression levels of KLF4 mRNA and protein were decreased in gastric cancer tissues compared with normal adjacent tissue. There was a dramatically inverse correlation between the expression levels of miR-10b and KLF4 mRNA in GC (r=-0.339, P=0.006). These findings indicate that miR-10b was upregulated in GC and may have a key role in GC pathogenesis and development through the downregulation of its target gene KLF4.

• Authors: Zhihong Ma, Yingrong Chen, Lishan Min, Liqin Li, Huilian Huang, Jing Li, Qiang Yan, Pengtao Song, Lichen Dai, Xing Yao
• Journal: International journal of clinical and experimental pathology (Int J Clin Exp Pathol) Vol. 8 Issue 5 Pg. 5071-9 ( 2015) ISSN: 1936-2625 [Electronic] United States
• PMID: 26191201 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 3' Untranslated Regions
• KLF4 protein, human
• Kruppel-Like Factor 4
• Kruppel-Like Transcription Factors
• MIRN10 microRNA, human
• MicroRNAs
• RNA, Messenger
• Luciferases

Topics

• 3' Untranslated Regions
• Binding Sites
• Carcinoma (genetics, metabolism, pathology)
• Cell Line, Tumor
• Down-Regulation
• Gene Expression Regulation, Neoplastic
• Genes, Reporter
• HEK293 Cells
• Humans
• Kruppel-Like Factor 4
• Kruppel-Like Transcription Factors (genetics, metabolism)
• Luciferases (genetics, metabolism)
• MicroRNAs (genetics, metabolism)
• RNA, Messenger (metabolism)
• Signal Transduction
• Stomach Neoplasms (genetics, metabolism, pathology)
• Transfection
• Up-Regulation