The Effect of Electroporation of a Lyotroic Liquid Crystal Genistein-Based Formulation in the Recovery of Murine Melanoma Lesions.

Abstract

A lamellar lyotropic liquid crystal genistein-based formulation (LLC-Gen) was prepared in order to increase the aqueous solubility of the lipophilic phytocompound genistein. The formulation was applied locally, in a murine model of melanoma, with or without electroporation. The results demonstrated that, when the formulation was applied by electroporation, the tumors appeared later. During the 21 days of the experiment, the LLC-Gen formulation decreased the tumor volume, the amount of melanin and the degree of erythema, but when electroporation was applied, all these parameters indicated a better prognosis even (lower tumor volume, amount of melanin and degree of erythema). Although hematoxylin-eosin (HE) staining confirmed the above events, application of the LLC-Gen formulation by electroporation did not lead to a significant effect in terms of the serum concentrations of the protein S100B and serum neuron specific enolase (NSE), or the tissue expression of the platelet-derived growth factor receptor β (PDGFRβ) antibody.

• Authors: Corina Danciu, Szilvia Berkó, Gábor Varju, Boglárka Balázs, Lajos Kemény, István Balázs Németh, Andreea Cioca, Alexandra Petruș, Cristina Dehelean, Citu Ioan Cosmin, Elena Amaricai, Claudia Crina Toma
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 16 Issue 7 Pg. 15425-41 (Jul 08 2015) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 26184156 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anticarcinogenic Agents
• Drug Carriers
• S100 Calcium Binding Protein beta Subunit
• S100b protein, mouse
• Triazines
• Genistein
• Receptor, Platelet-Derived Growth Factor beta
• Phosphopyruvate Hydratase
• melamine

Topics

• Animals
• Anticarcinogenic Agents (administration & dosage, chemistry)
• Cell Line, Tumor
• Chemistry, Pharmaceutical
• Drug Carriers (chemistry)
• Electroporation (methods)
• Female
• Genistein (administration & dosage, chemistry)
• Immunohistochemistry
• Liquid Crystals (chemistry)
• Melanoma (drug therapy, metabolism, pathology)
• Mice
• Mice, Inbred C57BL
• Phosphopyruvate Hydratase (blood)
• Receptor, Platelet-Derived Growth Factor beta (metabolism)
• Rheology
• S100 Calcium Binding Protein beta Subunit (blood)
• Skin (metabolism, pathology)
• Transplantation, Homologous
• Triazines (metabolism)