Astrocyte-elevated gene-1 mediates insulin-like growth factor 1-induced the progression of cardiac myxoma.

Abstract

Recently, astrocyte-elevated gene-1 (AEG-1) and insulin-like growth factor 1 (IGF-1) have been involved in the regulation of multiple signaling pathways in tumorigenesis. To date, the detailed mechanisms underlying IGF-1-AEG-1 pathway-induced proliferation and apoptosis in cardiac myxoma (CM) was not reported. In the present study, we used immnohistochemistry, immunoblotting, and qRT-PCR to detect the expression profile of IGF-1 and AEG-1 in 90 CM tissues, and then cultured CM cells were subjected to si-AEG-1, in vitro, and in vivo assays. Our findings showed that IGF-1 and AEG-1 were obviously upregulated in CM tissues and markedly associated with tumor size. When CM cells were treated with si-AEG-1, si-AEG-1 attenuated IGF-1-induced CM cell growth and enhanced cell apoptosis. Mechanically, we validated the expression of AEG-1, p-Erk1/2, and p-Akt increased in CM cells in response to IGF-1 treatment in a time-dependent manner. However, si-AEG-1 affected the expression of these proteins. Functionally, we found the knockdown of AEG-1-inhibited G1/S transition and tumor formation of CM cells. In conclusion, AEG-1 regulates IGF-1-induced proliferation and apoptosis via Erk1/2 and Akt signaling in CM development, which suggests IGF-1-AEG-1 signaling could be recommended to be a useful target to exert anti-tumor effects on CM.

• Authors: Changcun Fang, Yuwen Shen, Peng Qi, Zhengqin Liu, Min Zhang, Xinyan Pang
• Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (Tumour Biol) Vol. 36 Issue 12 Pg. 9769-77 (Dec 2015) ISSN: 1423-0380 [Electronic] Netherlands
• PMID: 26156800 (Publication Type: Journal Article, Retracted Publication)

Chemical References

• Cell Adhesion Molecules
• IGF1 protein, human
• MTDH protein, human
• Membrane Proteins
• RNA-Binding Proteins
• Insulin-Like Growth Factor I
• Proto-Oncogene Proteins c-akt
• Mitogen-Activated Protein Kinase 3

Topics

• Adult
• Aged
• Animals
• Apoptosis
• Cell Adhesion Molecules (biosynthesis, genetics)
• Cell Line, Tumor
• Cell Proliferation (genetics)
• Disease Progression
• Female
• Gene Expression Regulation, Neoplastic
• Heart Neoplasms (genetics, pathology)
• Humans
• Insulin-Like Growth Factor I (biosynthesis, genetics)
• Male
• Membrane Proteins
• Mice
• Middle Aged
• Mitogen-Activated Protein Kinase 3 (genetics)
• Myxoma (genetics, pathology)
• Proto-Oncogene Proteins c-akt (genetics)
• RNA-Binding Proteins
• Xenograft Model Antitumor Assays