Salvianolic acid A inhibits endothelial dysfunction and vascular remodeling in spontaneously hypertensive rats.

Abstract

AIMS:

Despite the numerous pharmacological agents available for hypertension therapy, hypertension-related microvascular remodeling is not resolved, eventually leading to end-organ damage. The aim of the present study was to investigate the protection of salvianolic acid A (SalA) against microvascular remodeling in vitro and in vivo.

MAIN METHODS:

Spontaneously hypertensive rats (SHRs) were administered 2.5, 5 or 10 mg/kg SalA via intraperitoneal injection once a day for 4 weeks. The tail-cuff method was applied to monitor blood pressure; the microvascular structure of the retina was detected by hematoxylin-eosin and immunohistochemical staining; the function of mesenteric arteries was measured by DMT wire myography; endothelial cell proliferation was estimated using the Cell Counting Kit-8; endothelial cell migration was evaluated by wound healing and transwell assay; and endothelial cell integrity was detected by transendothelial electrical resistance and permeability assays.

KEY FINDINGS:

Although no antihypertensive effects of SalA were observed, SalA attenuated the microvascular inward remodeling of the retina and improved microvascular function in the mesenteries in vivo. Further cell experiments confirmed the beneficial effects of SalA on the integrity of the endothelial monolayer in vitro.

SIGNIFICANCE:

Salvianolic acid A inhibited endothelial dysfunction and vascular remodeling in spontaneously hypertensive rats. Therefore, salvianolic acid A could be a potential drug therapy to prevent further targeted organ damage induced by vascular remodeling.

• Authors: Fukang Teng, Ying Yin, Yajun Cui, Yanping Deng, Defang Li, Kenka Cho, Ge Zhang, Aiping Lu, Wanying Wu, Min Yang, Xuan Liu, De-an Guo, Jun Yin, Baohong Jiang
• Journal: Life sciences (Life Sci) Vol. 144 Pg. 86-93 (Jan 01 2016) ISSN: 1879-0631 [Electronic] Netherlands
• PMID: 26135625 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015. Published by Elsevier Inc.

Chemical References

• Caffeic Acids
• Lactates
• salvianolic acid A

Topics

• Animals
• Caffeic Acids (pharmacology)
• Cell Membrane Permeability (drug effects)
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Endothelium, Vascular (drug effects)
• Human Umbilical Vein Endothelial Cells (drug effects)
• Humans
• Injections, Intraperitoneal
• Lactates (pharmacology)
• Male
• Mesenteric Arteries (drug effects)
• Microcirculation (drug effects)
• Myocytes, Smooth Muscle (drug effects)
• Rats
• Rats, Inbred SHR
• Rats, Inbred WKY
• Retinal Vessels (drug effects)
• Vascular Remodeling (drug effects)
• Wound Healing (drug effects)