Abstract |
The optimal T-cell attributes for adoptive cancer immunotherapy are unclear. Recent clinical trials of ex vivo-expanded tumor-infiltrating lymphocytes indicated that differentiated T effector cells can elicit durable antitumor responses in some patients with cancer, with their antitumor activity tightly correlated with their persistence in the host. Thus, there is great interest in the definition of intrinsic biomarkers that can predict the conversion of short-lived tumor antigen-specific T effector cells into long-lived T memory cells. Long-term persistence of ex vivo-expanded tumor-specific CD8+ T effector clones has been reported in refractory metastatic melanoma patients after adoptive T-cell transfer. By using highly homogeneous clone populations from these preparations, we performed a comparative transcriptional profiling to define preinfusion molecular attributes that can be ascribed to an effector-to-memory transition. Through this route, we discovered that preinfusion T-cell clones that expressed the IL7 receptor (IL7R) and c-myc were more likely to persist longer after adoptive transfer to patients. The predictive value of these two biomarkers was strengthened by using IL7R protein, IL7-induced pSTAT5, and c-myc mRNA expression to prospectively identify human tumor-specific T effector clones capable of engraftment into immunodeficient mice. Overall, our findings reveal IL7R and c-myc expression as intrinsic biomarkers that can predict the fate of CD8+ T effector cells after adoptive transfer.
|
Authors | Smita S Chandran, Biman C Paria, Abhishek K Srivastava, Luke D Rothermel, Daniel J Stephens, Udai S Kammula |
Journal | Cancer research
(Cancer Res)
Vol. 75
Issue 16
Pg. 3216-26
(Aug 15 2015)
ISSN: 1538-7445 [Electronic] United States |
PMID | 26100671
(Publication Type: Journal Article, Research Support, N.I.H., Intramural)
|
Copyright | ©2015 American Association for Cancer Research. |
Chemical References |
- Il2rg protein, mouse
- Interleukin Receptor Common gamma Subunit
- MYC protein, human
- Proto-Oncogene Proteins c-myc
- Receptors, Interleukin-7
|
Topics |
- Adoptive Transfer
(methods)
- Adult
- Aged
- Animals
- CD8-Positive T-Lymphocytes
(immunology, metabolism, transplantation)
- Cell Line
- Cell Line, Tumor
- Female
- Gene Expression Regulation, Neoplastic
(immunology)
- Humans
- Immunologic Memory
(immunology)
- Immunotherapy, Adoptive
(methods)
- Interleukin Receptor Common gamma Subunit
(deficiency, genetics)
- Male
- Melanoma
(genetics, immunology, therapy)
- Mice, Inbred NOD
- Mice, Knockout
- Mice, SCID
- Middle Aged
- Oligonucleotide Array Sequence Analysis
- Proto-Oncogene Proteins c-myc
(genetics, immunology, metabolism)
- Receptors, Interleukin-7
(genetics, immunology, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Transcriptome
(immunology)
- Transplantation, Heterologous
|