Ameliorative Effect of Vicenin-2 and Scolymoside on TGFBIp-Induced Septic Responses.

Abstract	Transforming growth factor β-induced protein (TGFBIp) is an extracellular matrix protein whose expression in several cell types is greatly increased by TGF-β. TGFBIp is released by the human umbilical vein endothelial cells (HUVECs) and functions as a mediator of experimental sepsis. Cyclopia subternata is a medicinal plant commonly used in traditional medicine to relieve pain in biological processes. In this study, we investigated the antiseptic effects and underlying mechanisms of vicenin-2 and scolymoside, two active compounds in C. subternata against TGFBIp-mediated septic responses in HUVECs and mice. The anti-inflammatory activities of vicenin-2 or scolymoside were determined by measuring permeability, human neutrophils adhesion and migration, and activation of pro-inflammatory proteins in TGFBIp-activated HUVECs and mice. According to the results, vicenin-2 or scolymoside effectively inhibited lipopolysaccharide-induced release of TGFBIp and suppressed TGFBIp-mediated septic responses, such as hyperpermeability, adhesion and migration of leukocytes, and expression of cell adhesion molecules. In addition, vicenin-2 or scolymoside suppressed the production of tumor necrosis factor-α and interleukin 6 and activation of nuclear factor-κB and extracellular regulated kinases 1/2 by TGFBIp. Vicenin-2 or scolymoside reduced cecal ligation and puncture (CLP)-induced septic mortality and pulmonary injury. Collectively, these results indicate that vicenin-2 and scolymoside could be a potential therapeutic agent for treatment of various severe vascular inflammatory diseases via inhibition of the TGFBIp signaling pathway.
Authors	Wonhwa Lee, Sae-Kwang Ku, Jong-Sup Bae
Journal	Inflammation (Inflammation) Vol. 38 Issue 6 Pg. 2166-77 (Dec 2015) ISSN: 1573-2576 [Electronic] United States
PMID	26084452 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Anti-Inflammatory Agents Cell Adhesion Molecules Extracellular Matrix Proteins Glucosides Inflammation Mediators Plant Extracts Transforming Growth Factor beta apigenin-6,8-di-C-glycopyranoside scolymoside betaIG-H3 protein Apigenin Extracellular Signal-Regulated MAP Kinases Luteolin
Topics	Animals Anti-Inflammatory Agents (isolation & purification, pharmacology) Apigenin (isolation & purification, pharmacology) Capillary Permeability (drug effects) Cell Adhesion (drug effects) Cell Adhesion Molecules (metabolism) Cells, Cultured Chemotaxis, Leukocyte (drug effects) Cyclopia Plant (chemistry) Disease Models, Animal Dose-Response Relationship, Drug Enzyme Activation Extracellular Matrix Proteins (immunology, metabolism) Extracellular Signal-Regulated MAP Kinases (metabolism) Glucosides (isolation & purification, pharmacology) Human Umbilical Vein Endothelial Cells (drug effects, immunology, metabolism) Inflammation Mediators (metabolism) Luteolin (isolation & purification, pharmacology) Male Mice, Inbred C57BL Neutrophils (drug effects, immunology, metabolism) Phytotherapy Plant Extracts (isolation & purification, pharmacology) Plants, Medicinal Pulmonary Edema (immunology, prevention & control) Sepsis (immunology, metabolism, microbiology, prevention & control) Time Factors Transforming Growth Factor beta (immunology, metabolism)

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