YGS40, an active fraction of Yi-Gan San, reduces hydrogen peroxide-induced apoptosis in PC12 cells.

Abstract

In our previous study, we have elucidated the chemical profile of YGS40, a fraction of Yi-Gan San (YGS), used for the treatment of Alzheimer's disease (AD). Oxidative stress-induced apoptosis is implicated in neurodegenerative disorders such as AD. The aim of the present study was to explore the protective effects of YGS40 against hydrogen peroxide (H2O2)-induced apoptosis in PC12 cells and the underlying mechanisms. PC12 cells were exposed to 100 μmol·L(-1) of H2O2 for 12 h with or without YGS40 pretreatment. Cytotoxicity was determined by MTT (3, (4, 5-dimethylthiazole-2-yl) 2, 5-diphenyl-tetrazolium bromide) and lactate dehydrogenase (LDH) release assays; apoptosis was detected by Annexin V/propidium iodide coupled staining and by determining caspase-3 activity and Bax and Bcl-2 protein levels. Mitochondrial membrane potential (MMP) was assessed by the retention of rhodamine123; and the activities of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured using commercially available enzymatic kits. Pretreatment with YGS40 significantly prevented H2O2-induced cytotoxicity and protected the cells against H2O2-triggered apoptosis characterized by externalization of membrane phosphatidylserine and caspase-3 activation and the increased ratio of Bax/Bcl-2 in PC12 cells. Further studies showed that YGS40 suppressed H2O2-induced MMP loss, increased SOD activity, and decreased MDA level. These findings suggest that YGS40 may be beneficial for the prevention and treatment of oxidative stress-mediated disorders.

• Authors: Yu-Rong Zhao, Wei Qu, Wen-Yuan Liu, Hao Hong, Feng Feng, Han Chen, Ning Xie
• Journal: Chinese journal of natural medicines (Chin J Nat Med) Vol. 13 Issue 6 Pg. 438-44 (Jun 2015) ISSN: 1875-5364 [Electronic] China
• PMID: 26073340 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.

Chemical References

• Antioxidants
• Drugs, Chinese Herbal
• Neuroprotective Agents
• Reactive Oxygen Species
• Yi-Gan San
• Malondialdehyde
• Hydrogen Peroxide
• Caspase 3

Topics

• Animals
• Antioxidants (pharmacology)
• Apoptosis (drug effects)
• Caspase 3 (metabolism)
• Cell Survival (drug effects)
• Drugs, Chinese Herbal (pharmacology)
• Hydrogen Peroxide (toxicity)
• Malondialdehyde (metabolism)
• Mitochondria (drug effects, enzymology, metabolism)
• Neuroprotective Agents (pharmacology)
• Oxidative Stress (drug effects)
• PC12 Cells
• Rats
• Reactive Oxygen Species (metabolism)