Altered expression of P2Y2 and P2X7 purinergic receptors in the isolated rat heart mediates ischemia-reperfusion injury.

Abstract	The aim of this study is to analyze the expression of purinergic receptors in the heart after ischemia-reperfusion, and their possible role in ischemia-reperfusion injury. Rat hearts were perfused according to the Langendorff technique and subjected to 30 min ischemia followed by 15 min reperfusion. Ischemia-reperfusion reduced the gene expression and protein content of purinergic receptors of the P2Y2 subtype, and increased the gene expression and protein content of the P2X7 subtype. Treatment with the agonist of the P2Y2 subtype 2-thio-UTP and with the antagonist of the P2X7 subtype Brilliant Blue improved myocardial function parameters, reduced cell death and increased the myocardial expression of antiapoptotic markers after ischemia-reperfusion. These results suggest that the myocardial expression of the protective P2Y2 subtype of purinergic receptors is reduced, whereas that of the harmful subtype P2X7 subtype is increased during coronary ischemia-reperfusion. This may contribute to myocardial injury in this condition.
Authors	Miriam Granado, Sara Amor, Juan José Montoya, Luis Monge, Nuria Fernández, Ángel Luis García-Villalón
Journal	Vascular pharmacology (Vascul Pharmacol) Vol. 73 Pg. 96-103 (Oct 2015) ISSN: 1879-3649 [Electronic] United States
PMID	26070527 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright	Copyright © 2015 Elsevier Inc. All rights reserved.
Chemical References	Apoptosis Regulatory Proteins P2rx7 protein, rat P2ry2 protein, rat Purinergic P2X Receptor Antagonists Purinergic P2Y Receptor Agonists Receptors, Purinergic P2X7 Receptors, Purinergic P2Y2
Topics	Animals Apoptosis Apoptosis Regulatory Proteins (metabolism) Down-Regulation Hemodynamics Isolated Heart Preparation Male Myocardial Reperfusion Injury (drug therapy, genetics, metabolism, pathology, physiopathology) Myocardium (metabolism, pathology) Purinergic P2X Receptor Antagonists (pharmacology) Purinergic P2Y Receptor Agonists (pharmacology) Rats, Sprague-Dawley Receptors, Purinergic P2X7 (drug effects, genetics, metabolism) Receptors, Purinergic P2Y2 (drug effects, genetics, metabolism) Signal Transduction Time Factors Up-Regulation

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