Inhibition of SHP-2 promotes radiosensitivity in glioma.

Abstract

As a phosphatase, SHP-2 has been identified to be involved in regulating several cell functions, including growth, division, adhesion and motility. Therefore, SHP‑2 may affect the response of glioma to radiotherapy, such as via enhancing angiogenesis. The present study aimed to investigate the function of SHP‑2, a protein tyrosine phosphatase, in the radiosensitivity of glioma. U251, U87 and SHG44 glioma cell lines were transfected with small interfering (si)RNA against SHP‑2 and cell proliferation was assessed using a cell counting kit 8 assay, cell apoptosis was assessed by fluorescence‑activated cell sorting and immunoblotting, cell invasion was determined by an invasion assay, and the vasculogenic mimicry capacity was assessed by a tube formation assay. SHP‑2 siRNA transfection reduced the proliferation and increased apoptosis in the glioma cell lines. Downregulation of SHP‑2 suppressed glioma cell invasion and vasculogenic mimicry. These results demonstrated that no significant difference was observed between glioma tissues and normal brain tissues, however, silencing of SHP‑2 inhibited cell proliferation, invasion and vasculogenic mimicry in the glioma cell lines. SHP‑2 may be a novel therapeutic target for glioma.

• Authors: Quanye Sun, Luyan Mu, Wanchen Qiao, Hui Li, Jiabin Tang, Ce Wang, Wei Hu, Tianshu Zhao, Baijing Dong, Yuwen Song, Xiaoqian Liu
• Journal: Molecular medicine reports (Mol Med Rep) Vol. 12 Issue 3 Pg. 3563-3568 (Sep 2015) ISSN: 1791-3004 [Electronic] Greece
• PMID: 26004555 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Small Interfering
• PTPN11 protein, human
• Protein Tyrosine Phosphatase, Non-Receptor Type 11

Topics

• Adolescent
• Adult
• Aged
• Brain (blood supply, metabolism, pathology)
• Brain Neoplasms (blood supply, genetics, radiotherapy, therapy)
• Cell Line, Tumor
• Female
• Glioma (blood supply, genetics, radiotherapy, therapy)
• Humans
• Male
• Middle Aged
• Protein Tyrosine Phosphatase, Non-Receptor Type 11 (genetics)
• RNA, Small Interfering (genetics, therapeutic use)
• RNAi Therapeutics
• Transfection
• Young Adult