Marine lipopeptide Iturin A inhibits Akt mediated GSK3β and FoxO3a signaling and triggers apoptosis in breast cancer.

Abstract

Akt kinase is a critical component of the PI3K/Akt signaling pathway, which is frequently over expressed in human cancers including breast. Therapeutic regimens for inhibiting breast cancer with aberrant Akt activity are essential. Here, we evaluated antitumor effect of a marine bacteria derived lipopeptide 'Iturin A' on human breast cancer in vitro and in vivo through disrupting Akt pathway. Proliferation of MDA-MB-231 and MCF-7 breast cancer cells were significantly inhibited by Iturin A and it induced apoptosis as confirmed by increased Sub G1 populations, DNA fragmentation, morphological changes and western blot analysis. Furthermore, Iturin A inhibited EGF induced Akt phosphorylation (Ser473 and Thr308) and its downstream targets GSK3β and FoxO3a. Iturin A inactivated MAPK as well as Akt kinase leading to the translocation of FoxO3a to the nucleus. Gene silencing of Akt in MDA-MB-231 and MCF-7 cells reduced the sensitivity of cancer cells to Iturin A. Interestingly, overexpression of Akt with Akt plasmid in cancer cells caused highly susceptible to induce apoptosis by Iturin A treatment. In a xenograft model, Iturin A inhibited tumor growth with reduced expressions of Ki-67, CD-31, P-Akt, P-GSK3β, P-FoxO3a and P-MAPK. Collectively, these findings imply that Iturin A has potential anticancer effect on breast cancer.

• Authors: Goutam Dey, Rashmi Bharti, Gunaseelan Dhanarajan, Subhasis Das, Kaushik Kumar Dey, B N Prashanth Kumar, Ramkrishna Sen, Mahitosh Mandal
• Journal: Scientific reports (Sci Rep) Vol. 5 Pg. 10316 (May 14 2015) ISSN: 2045-2322 [Electronic] England
• PMID: 25974307 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• FOXO3 protein, human
• Forkhead Box Protein O3
• Forkhead Transcription Factors
• Peptides, Cyclic
• iturin A
• GSK3B protein, human
• Glycogen Synthase Kinase 3 beta
• Gsk3b protein, mouse
• Proto-Oncogene Proteins c-akt
• Glycogen Synthase Kinase 3

Topics

• Animals
• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Bacillus megaterium (metabolism)
• Breast Neoplasms (drug therapy)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Female
• Forkhead Box Protein O3
• Forkhead Transcription Factors (antagonists & inhibitors)
• Gene Expression Regulation, Neoplastic
• Glycogen Synthase Kinase 3 (antagonists & inhibitors)
• Glycogen Synthase Kinase 3 beta
• Humans
• MCF-7 Cells
• Mice
• Mice, Inbred BALB C
• Peptides, Cyclic (pharmacology)
• Proto-Oncogene Proteins c-akt (antagonists & inhibitors)
• Signal Transduction (drug effects)
• Transplantation, Heterologous
• Xenograft Model Antitumor Assays