[Comparative analysis of natural and synthetic antimutagens as regulators of gene expression in human cells under exposure to ionizing radiation].

Abstract

This paper studies the effect of plant peptides of thionine Ns-W2 extracted from seeds of fennel flower (Nigella sativa) and β-purothionine from wheat germs (Triticum kiharae), as well as a synthetic antimutagen (crown-compound), on the expression of several genes involved in the.control of cellular homeostasis, processes of carcinogenesis, and radiation response in human rhabdomyosarcoma cells (RD cells), T-lymphoblastoid cell line Jurkat, and blood cells. All of these agents acted as antimutagens-anticarcinogens, reducing the expression of genes involved in carcinogenesis (genes of families MMP, TIMP, and IAP and G-protein genes) in a tumor cell. A pronounced reduction in the mRNA level of these genes was caused by thionine Ns-W2, and the least effect was demonstrated by β-purothionine. Antimutagens had very little effect on the mRNA levels of the several studied genes in normal blood cells.

• Authors: V F Mikhailov, A A Shishkina, I M Vasilyeva, L V Shulenina, N F Raeva, E A Rogozhin, M I Startsev, G D Zasukhina, S P Gromov, M V Alfimov
• Journal: Genetika (Genetika) Vol. 51 Issue 2 Pg. 147-55 (Feb 2015) ISSN: 0016-6758 [Print] Russia (Federation)
• PMID: 25966580 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antimutagenic Agents
• Peptides
• Phenothiazines
• Plant Extracts
• TP53 protein, human
• Tumor Suppressor Protein p53
• thionine

Topics

• Antimutagenic Agents (administration & dosage, chemistry)
• Carcinogenesis (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Nigella sativa (chemistry)
• Peptides (administration & dosage, chemistry)
• Phenothiazines (administration & dosage, chemistry)
• Plant Extracts (administration & dosage, chemistry)
• Radiation, Ionizing
• Rhabdomyosarcoma (drug therapy, genetics, pathology)
• Triticum (chemistry)
• Tumor Suppressor Protein p53 (biosynthesis)