Expression of miRNAs as Important Element of Melanoma Cell Plasticity in Response to Microenvironmental Stimuli.

Abstract

BACKGROUND:

Melanoma cells form monolayers in serum-containing media, however, in serum-free media they form anchorage-independent spheroids. We investigated miRNAs differentially expressed between these culture types and identified those that possibly control the plasticity of melanoma cells.

MATERIALS AND METHODS:

The expression of miRNAs in melanoma cells was evaluated with microarrays, and certain miRNAs were validated with real-time PCR. Several bioinformatic tools were used to assess the involvement of identified miRNAs in cancer-related pathways, and to compile the results of mRNA microarray data from the same melanoma cells.

RESULTS:

A total of 19 miRNAs were differentially expressed between monolayers and spheroids. miRNAs up-regulated in spheroids modulated cell motility and migration, whereas those up-regulated in monolayers suppressed melanogenesis.

CONCLUSION:

The present study identified those miRNAs that participate in the regulation of melanoma cell plasticity.

• Authors: Michal Wozniak, Malgorzata Sztiller-Sikorska, Malgorzata Czyz
• Journal: Anticancer research (Anticancer Res) Vol. 35 Issue 5 Pg. 2747-58 (May 2015) ISSN: 1791-7530 [Electronic] Greece
• PMID: 25964554 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

Chemical References

• Biomarkers, Tumor
• Culture Media, Serum-Free
• MicroRNAs
• RNA, Messenger

Topics

• Biomarkers, Tumor (biosynthesis, genetics)
• Cell Line, Tumor
• Culture Media, Serum-Free
• Gene Expression Regulation, Neoplastic (genetics)
• Humans
• Melanoma (genetics, pathology)
• MicroRNAs (biosynthesis, genetics)
• RNA, Messenger (biosynthesis)
• Tumor Microenvironment (genetics)