Monitoring the quality consistency of Weibizhi tablets by micellar electrokinetic chromatography fingerprints combined with multivariate statistical analyses, the simple quantified ratio fingerprint method, and the fingerprint-efficacy relationship.

Abstract

Micellar electrokinetic chromatography fingerprinting combined with quantification was successfully developed and applied to monitor the quality consistency of Weibizhi tablets, which is a classical compound preparation used to treat gastric ulcers. A background electrolyte composed of 57 mmol/L sodium borate, 21 mmol/L sodium dodecylsulfate and 100 mmol/L sodium hydroxide was used to separate compounds. To optimize capillary electrophoresis conditions, multivariate statistical analyses were applied. First, the most important factors influencing sample electrophoretic behavior were identified as background electrolyte concentrations. Then, a Box-Benhnken design response surface strategy using resolution index RF as an integrated response was set up to correlate factors with response. RF reflects the effective signal amount, resolution, and signal homogenization in an electropherogram, thus, it was regarded as an excellent indicator. In fingerprint assessments, simple quantified ratio fingerprint method was established for comprehensive quality discrimination of traditional Chinese medicines/herbal medicines from qualitative and quantitative perspectives, by which the quality of 27 samples from the same manufacturer were well differentiated. In addition, the fingerprint-efficacy relationship between fingerprints and antioxidant activities was established using partial least squares regression, which provided important medicinal efficacy information for quality control. The present study offered an efficient means for monitoring Weibizhi tablet quality consistency.

• Authors: Yingchun Liu, Guoxiang Sun, Yan Wang, Lanping Yang, Fangliang Yang
• Journal: Journal of separation science (J Sep Sci) Vol. 38 Issue 12 Pg. 2174-81 (Jun 2015) ISSN: 1615-9314 [Electronic] Germany
• PMID: 25867134 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Antioxidants
• Borates
• Drugs, Chinese Herbal
• Electrolytes
• Plant Extracts
• Tablets
• weibizhi
• Sodium Dodecyl Sulfate
• Sodium Hydroxide
• sodium borate

Topics

• Antioxidants (chemistry)
• Borates (chemistry)
• Chromatography, Micellar Electrokinetic Capillary
• Drugs, Chinese Herbal (chemistry)
• Electrolytes
• Least-Squares Analysis
• Multivariate Analysis
• Plant Extracts (chemistry)
• Quality Control
• Reproducibility of Results
• Sodium Dodecyl Sulfate (chemistry)
• Sodium Hydroxide (chemistry)
• Tablets (chemistry)