Comparative analysis of the expression of E-cadherin, β-catenin, and β1 integrin in congenital and acquired cholesteatoma.

Abstract

E-cadherin, β-catenin, and β1 integrin are important cell adhesion molecules to maintain epithelial structure and function. We investigated the expression of these cell adhesion molecules in cholesteatomas to understand the role of cell-cell and cell-extracellular matrix interaction in cholesteatomas. An immunohistochemical investigation was carried out on 35 cholesteatoma tissue samples (14 congenital, 21 acquired cholesteatomas) and 10 normal retroauricular skin (RAS) tissues which are obtained during middle ear surgery. The expression rate was measured to find out differences between retroauricular skin and cholesteatoma, as well as between congenital and acquired cholesteatoma. E-cadherin expression rate was significantly lower in the cholesteatoma (spinous layer 88.7 ± 17.9 %, granular layer 54.6 ± 22.6 %) than in the RAS (100 %, 74.4 ± 7.4 %) and in the acquired (83.3 ± 19.4 %, 48.1 ± 22.9 %) than in the congenital (96.7 ± 12.0 %, 64.4 ± 18.8 %). β-catenin expression rate was significantly lower in the cholesteatoma (spinous layer 84.1 ± 17.2 %, granular layer 28.7 ± 30.8 %) than in the RAS (100 %, 75.9 ± 6.1 %) and in the acquired (78.1 ± 17.0 %, 17.1 ± 22.3 %) than in the congenital (93.2 ± 13.5 %, 46.1 ± 34.2 %). The expression pattern of β-catenin is similar to that of E-cadherin. In β1 integrin, there was no significant difference of the expression rate between RAS and cholesteatoma, as well as between congenital and acquired cholesteatoma. In conclusion, the expression of E-cadherin and β-catenin is reduced in cholesteatoma, and the reduction is more pronounced in acquired cholesteatoma than in congenital cholesteatoma. Acquired cholesteatomas showed more aggressive characteristics than congenital cholesteatomas in terms of cell-cell adhesion.

• Authors: Dong Wook Lee, Jae Ho Chung, Seung Hwan Lee, Chul Won Park, Sung-Ho Kang, Young Ha Oh, Ju Yeon Pyo
• Journal: European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery (Eur Arch Otorhinolaryngol) Vol. 273 Issue 4 Pg. 845-51 (Apr 2016) ISSN: 1434-4726 [Electronic] Germany
• PMID: 25864182 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cadherins
• Cell Adhesion Molecules
• Integrin beta1
• beta Catenin
• RNA

Topics

• Adolescent
• Adult
• Aged
• Cadherins (biosynthesis, genetics)
• Cell Adhesion Molecules
• Child
• Child, Preschool
• Cholesteatoma (congenital, genetics, metabolism, pathology)
• Cholesteatoma, Middle Ear (genetics, metabolism, pathology)
• Female
• Gene Expression Regulation
• Humans
• Immunohistochemistry
• Infant
• Integrin beta1 (biosynthesis, genetics)
• Male
• Middle Aged
• RNA (genetics)
• Young Adult
• beta Catenin (biosynthesis, genetics)