Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: AIM OF THE STUDY: MATERIALS AND METHODS: Forty male ICR mice were administered with 1, 2-dimethyl-hydrazine ( DMH) and dextran sodium sulfate (DSS). Ten mice were given no further treatment, the rest were administered with different doses of TTS (5, 10, 20mg/kg) orally, every three days from the 9th week to the 20th week. RESULTS: TTS effectively protected ICR mice against DMH/DSS-induced tumorigenesis. The incidence of tumor development was 90% (9/10) in the mice treated with DMH/DSS, but that was reduced to 50% (5/10), 40% (4/10), and 20% (2/10), respectively, in the mice treated with 5%, 10%, and 20% of TTS. Results of Ki-67 staining, TUNEL assay and caspase-3 activity assay revealed that TTS moderately decreased abnormal proliferation and increased apoptosis of colonic epithelial cells. It inhibited the growth and triggered the apoptosis of HT-29 cells, partly through suppressing mitogen-actived protein kinases (MAPKs) and triggering mitochondrial-mediated apoptotic pathway. Three compounds, namely, Paris saponin VII, polyphylloside III and Paris saponin VI, were important active compounds in TTS. CONCLUSION: These data suggest that TTS has a potential role in clinical prevention and treatment for colorectal cancer.
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Authors | Yuhua Li, Changxu Liu, Dan Xiao, Jing Han, Zhenggang Yue, Yang Sun, Lei Fan, Feng Zhang, Jin Meng, Rong Zhang, Zhipeng Wang, Qibing Mei, Aidong Wen |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 168
Pg. 136-45
(Jun 20 2015)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 25849732
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Antineoplastic Agents, Phytogenic
- Saponins
- Dextran Sulfate
- Mitogen-Activated Protein Kinases
- Casp3 protein, mouse
- Caspase 3
- 1,2-Dimethylhydrazine
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Topics |
- 1,2-Dimethylhydrazine
- Animals
- Antineoplastic Agents, Phytogenic
(pharmacology, therapeutic use)
- Apoptosis
(drug effects)
- Caspase 3
(metabolism)
- Cell Proliferation
(drug effects)
- Colitis
(chemically induced, complications, metabolism)
- Colorectal Neoplasms
(drug therapy, etiology, metabolism)
- Dextran Sulfate
- HT29 Cells
- Humans
- Male
- Membrane Potential, Mitochondrial
(drug effects)
- Mice, Inbred ICR
- Mitogen-Activated Protein Kinases
(metabolism)
- Saponins
(pharmacology, therapeutic use)
- Trillium
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