Combined effect of microRNA, nutraceuticals and drug on pancreatic cancer cell lines.

Abstract

AIM:

We proposed to investigate the combination effect of microRNA, nutraceuticals and drug (MND), in two pancreatic cancer cell lines to assess the therapeutic potential.

MATERIALS AND METHODS:

MIA PaCa-2 and PANC-1 cells transfected with miR-101 or miR-24-2 were treated with Betulinic acid or Thymoquinone and gemcitabine independently and in combination and assessed for the extent of synergism in both experimental and control conditions, considering significance at the p value of <0.05.

RESULTS:

miR-101 or miR-24-2 over-expressing cells when treated with lower than IC50 doses of the dietary compounds and drug showed a reduced (37-50%) viability in two cell lines with differential synergistic effect and the outcome for Pro-caspase3, Poly (ADP-ribose) polymerase (PARP) cleavage and PKM2 expression.

CONCLUSION:

Two independent microRNA backgrounds showed promise in therapeutic intervention of gemcitabine sensitive, MIA PaCa-2 and resistant, PANC-1 pancreatic cancer cells, in combination with dietary agents and drug.

• Authors: Archana Pandita, Siddharth Manvati, Shashank K Singh, Samantha Vaishnavi, Rameshwar N K Bamezai
• Journal: Chemico-biological interactions (Chem Biol Interact) Vol. 233 Pg. 56-64 (May 25 2015) ISSN: 1872-7786 [Electronic] Ireland
• PMID: 25841339 (Publication Type: Journal Article)
• Copyright: Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Antimetabolites, Antineoplastic
• Benzoquinones
• MIRN101 microRNA, human
• MIRN24 microRNA, human
• MicroRNAs
• Pentacyclic Triterpenes
• Triterpenes
• Deoxycytidine
• thymoquinone
• Betulinic Acid
• Gemcitabine

Topics

• Antimetabolites, Antineoplastic (pharmacology)
• Apoptosis (drug effects)
• Benzoquinones (pharmacology)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Deoxycytidine (analogs & derivatives, pharmacology)
• Dietary Supplements (analysis)
• Drug Synergism
• Humans
• MicroRNAs (genetics)
• Pancreas (drug effects, metabolism, pathology)
• Pancreatic Neoplasms (drug therapy, genetics, pathology)
• Pentacyclic Triterpenes
• Transfection
• Triterpenes (pharmacology)
• Betulinic Acid
• Gemcitabine