Effects of down-regulated steroidogenic factor-1 on ACTH and potassium chloride-induced steroid synthesis in H295R cells.

Abstract	The prevalence of adrenal diseases in the cortex is more common than that in the medulla in the form of hormone disorder or neoplasm. Steroidogenic factor—1 (SF—1) is important in regulating aldosterone synthase (CYP11B2) and cortisol synthase (CYP11B1). SF—1 is increased in aldosterone—producing adenoma (APA) and cortisol—producing adenoma (CPA). Overexpression of SF—1 has been extensively studied, but the available in—depth information regarding the effects of downregulated SF—1 on CYP11B2/CYP11B1 and their regulators is limited. In this paper, we attempted to investigate the effects of downregulated SF—1 on aldosterone to adrenocorticotropic hormone (ACTH) and potassium chloride (KCl) stimulation and those on cortisol to ACTH stimulation through RNA interference in acute and chronic phases. Downregulated SF—1 decreased the sensitivity of aldosterone to ACTH/KCl and that of cortisol to ACTH stimulation. This study provides new insights into the influence of SF—1 on adrenocortical diseases by considering the effects of SF—1 on regulation.
Authors	D Hu, X Wang, R Cao, X Ding
Journal	Cellular and molecular biology (Noisy-le-Grand, France) (Cell Mol Biol (Noisy-le-grand)) Vol. 61 Issue 1 Pg. 51-5 (Mar 28 2015) ISSN: 1165-158X [Electronic] France
PMID	25817346 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	RNA, Small Interfering Steroidogenic Factor 1 Steroids Aldosterone Potassium Chloride Adrenocorticotropic Hormone Cyclic AMP Cytochrome P-450 CYP11B2 Steroid 11-beta-Hydroxylase Hydrocortisone
Topics	Adrenal Cortex (cytology, drug effects, metabolism) Adrenocorticotropic Hormone (pharmacology) Aldosterone (metabolism) Cell Line Cyclic AMP (metabolism) Cytochrome P-450 CYP11B2 (metabolism) Down-Regulation (drug effects, physiology) Humans Hydrocortisone (metabolism) In Vitro Techniques Potassium Chloride (pharmacology) RNA Interference RNA, Small Interfering (pharmacology) Signal Transduction (physiology) Steroid 11-beta-Hydroxylase (metabolism) Steroidogenic Factor 1 (antagonists & inhibitors, genetics, metabolism) Steroids (metabolism)

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