Abstract | BACKGROUND: It is well accepted that type 2 diabetic mellitus (T2DM) results in the poor outcome of ischemic stroke. However, the mechanisms by which T2DM causes aggravated cerebral ischemic/reperfusion (I/R) injury are not clear. Recently, endothelial progenitor cells (EPCs) are considered to be related with the outcome of ischemic stroke. More importantly, T2DM can affect the function of circulating EPCs. This study tried to investigate whether T2DM worsens the cerebral I/R injury via affecting circulating EPCs. METHODS: RESULTS: We found that diabetic rats subjected to I/R exhibited significantly severe deterioration in neurologic deficits compared with nondiabetic counterparts, which manifested higher infarct volume and cell apoptosis as well as lower neurologic defective score. There was no significant difference on the plasma glucose of groups before cerebral I/R injury compared with that of the groups posterior to cerebral I/R injury despite cerebral I/R injury had the tendency to increase the plasma glucose no matter in the presence or the absence of T2DM. In addition, there were the marked downregulation of circulating EPCs counts and the levels of VEGF and eNOS in diabetic rats before the cerebral I/R injury. Despite I/R injury without T2DM, there was a significant increase in the circulating EPCs counts, the circulating EPCs counts in I/R injury with T2DM group were significantly decreased compared with those in the other 3 groups. We also observed that the level of eNOS was significantly improved by I/R injury without considering the presence of T2DM. CONCLUSIONS: Thus, our present study suggested that it might be the impaired EPCs mobilization into the blood that contributed to the worse outcome of cerebral I/R injury with T2DM.
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Authors | HongNa Yang, Shuang Ma, Ying Liu, Yi Li, Wei Wu, EnJi Han, GuoYong Jia, CuiLan Wang |
Journal | Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association
(J Stroke Cerebrovasc Dis)
Vol. 24
Issue 5
Pg. 980-7
(May 2015)
ISSN: 1532-8511 [Electronic] United States |
PMID | 25813059
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 National Stroke Association. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Vascular Endothelial Growth Factor A
- Streptozocin
- Nitric Oxide Synthase Type III
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Topics |
- Animals
- Brain Ischemia
(complications)
- Cell Movement
(physiology)
- Diabetes Mellitus, Experimental
(complications, pathology)
- Diet, High-Fat
(adverse effects)
- Disease Models, Animal
- Endothelial Progenitor Cells
(physiology)
- Flow Cytometry
- In Situ Nick-End Labeling
- Male
- Neurologic Examination
- Nitric Oxide Synthase Type III
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(complications)
- Streptozocin
(toxicity)
- Stroke
(complications, etiology)
- Vascular Endothelial Growth Factor A
(metabolism)
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