Modulation of gene expression in Escherichia coli infected with single-stranded bacteriophage phi X174.

Abstract

Synthesis of tryptophanase, D-serine deaminase and alkaline phosphatase in Escherichia coli C was repressed as the result of infection with the single-stranded DNA bacteriophage phi X174. However, the degree of repression differed, the more catabolite-sensitive the operon was, the more severe was the repression. For the catabolite-sensitive enzymes it was found that cyclic adenosine 3'5' monophosphate (cyclic AMP or cAMP) was unable to release or reduce the phage-induced inhibition. Experiments with amber mutants of phi X174 revealed that A, product of cistron A, was responsible for the inhibition. The cistron A product probably acted at the level of transcription. The possible role of A in the observed modulation of gene expression is discussed.

• Authors: A Ghosh, S K Pal, R K Poddar
• Journal: Molecular & general genetics : MGG (Mol Gen Genet) Vol. 198 Issue 2 Pg. 304-8 ( 1985) ISSN: 0026-8925 [Print] Germany
• PMID: 2580215 (Publication Type: Journal Article)

Chemical References

• RNA, Bacterial
• RNA, Messenger
• Repressor Proteins
• Transcription Factors
• Cyclic AMP
• Alkaline Phosphatase
• Tryptophanase
• L-Serine Dehydratase

Topics

• Alkaline Phosphatase (genetics)
• Bacteriophage phi X 174 (genetics)
• Cyclic AMP (physiology)
• Escherichia coli (genetics)
• Gene Expression Regulation
• L-Serine Dehydratase (genetics)
• RNA, Bacterial (genetics)
• RNA, Messenger (genetics)
• Repressor Proteins (genetics)
• Transcription Factors (genetics)
• Transcription, Genetic
• Tryptophanase (genetics)