Antiproliferative effect of isolated isoquinoline alkaloid from Mucuna pruriens seeds in hepatic carcinoma cells.

Abstract

The present study was undertaken to investigate the antiproliferative action of isolated M1 (6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid) from Mucuna pruriens seeds using human hepatic carcinoma cell line (Huh-7 cells). Initially, docking studies was performed to find out the binding affinities of M1 to caspase-3 and 8 enzymes. Later, cytotoxic action of M1 was measured by cell growth inhibition (MTT), followed by caspase-3 and 8 enzymes assay colorimetrically. Our results collectively suggested that M1 had strong binding affinity to caspase-8 in molecular modelling. M1 possessed antiproliferative activity on Huh-7 cells (EC50 = 13.97 μM) and also inhibited the action of caspase-8 enzyme, signified process of apoptosis. M1 was active against Huh-7 cells that may be useful for future hepatic cancer treatment.

• Authors: Pranesh Kumar, Atul Rawat, Amit K Keshari, Ashok K Singh, Siddhartha Maity, Arnab De, Amalesh Samanta, Sudipta Saha
• Journal: Natural product research (Nat Prod Res) Vol. 30 Issue 4 Pg. 460-3 ( 2016) ISSN: 1478-6427 [Electronic] England
• PMID: 25774560 (Publication Type: Journal Article)

Chemical References

• 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid
• Alkaloids
• Isoquinolines
• Tetrahydroisoquinolines
• CASP3 protein, human
• CASP8 protein, human
• Caspase 3
• Caspase 8

Topics

• Alkaloids (isolation & purification, pharmacology)
• Apoptosis (drug effects)
• Carcinoma, Hepatocellular (pathology)
• Caspase 3 (metabolism)
• Caspase 8 (metabolism)
• Cell Line, Tumor (drug effects)
• Cell Proliferation (drug effects)
• Humans
• Isoquinolines (isolation & purification, pharmacology)
• Liver Neoplasms (pathology)
• Molecular Docking Simulation
• Molecular Structure
• Mucuna (chemistry)
• Seeds (chemistry)
• Tetrahydroisoquinolines (isolation & purification, pharmacology)