A recombinant canine distemper virus expressing a modified rabies virus glycoprotein induces immune responses in mice.

Abstract

Canine distemper virus (CDV) and rabies virus (RV) are two important pathogens of the dog. CDV, a member of the morbillivirus genus, has shown promise as an expression vector. The glycoprotein from RV is a main contributor to protective immunity and capable of eliciting the production of virus-neutralizing antibodies. In this study, we recovered an attenuated strain of canine distemper virus and constructed a recombinant virus, rCDV-RV-G, expressing a modified (R333Q) rabies virus glycoprotein (RV-G) of RV Flury strain LEP. RV-G expression by the recombinant viruses was confirmed. Furthermore, G was proved to be incorporated into the surface of CDV particles. While replication of the recombinant virus was slightly reduced compared with the parental CDV, it stably expressed the RV-G over ten serial passages. Inoculation of mice induced specific neutralizing antibodies against both RV-G and CDV. Therefore, the rCDV-RV-G has the potential as a vaccine that may be used to control rabies virus infection in dogs and other animals.

• Authors: Zhili Li, Jigui Wang, Daoli Yuan, Shuang Wang, Jiazeng Sun, Bao Yi, Qiang Hou, Yaping Mao, Weiquan Liu
• Journal: Virus genes (Virus Genes) Vol. 50 Issue 3 Pg. 434-41 (Jun 2015) ISSN: 1572-994X [Electronic] United States
• PMID: 25764477 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Neutralizing
• Antibodies, Viral
• Antigens, Viral
• Drug Carriers
• Glycoproteins
• Rabies Vaccines
• Recombinant Proteins
• Vaccines, Attenuated
• Vaccines, Synthetic
• Viral Envelope Proteins
• glycoprotein G, Rabies virus

Topics

• Animals
• Antibodies, Neutralizing (blood)
• Antibodies, Viral (blood)
• Antigens, Viral (genetics, immunology)
• Distemper Virus, Canine (genetics)
• Dogs
• Drug Carriers
• Glycoproteins (genetics, immunology)
• Mice
• Rabies Vaccines (administration & dosage, genetics, immunology)
• Rabies virus (genetics, immunology)
• Recombinant Proteins (genetics, immunology)
• Vaccines, Attenuated (administration & dosage, genetics, immunology)
• Vaccines, Synthetic (administration & dosage, genetics, immunology)
• Viral Envelope Proteins (genetics, immunology)