CD103+ Tumor Infiltrating Lymphocytes Predict a Favorable Prognosis in Urothelial Cell Carcinoma of the Bladder.

Abstract

PURPOSE:

CD8(+) TILs at different tumor sites have diverse clinical attributes, which might result from distinct tumor microenvironments that promote differentiation into distinct subsets. However, only a few markers have been identified that can define CD8(+) T-cell subsets. CD103 is a marker of tissue resident memory CD8(+) T cells. In this retrospective study we investigated the cellular source and clinical significance of CD103 expression in urothelial cell carcinoma of bladder tissues in situ.

MATERIALS AND METHODS:

Immunohistochemistry and immunofluorescence were used to identify the cellular source of CD103 in bladder urothelial cell carcinoma tissues. Kaplan-Meier analysis and Cox proportional hazards regression models were applied to estimate overall and recurrence-free survival in 302 patients with bladder urothelial cell carcinoma.

RESULTS:

CD8(+) T cells but not natural killer cells accounted for most CD103 expressing cells in bladder urothelial cell carcinoma tissues. Notably CD103(+) cells were predominantly located in intratumor regions rather than in associated stroma (p < 0.0001). The density of intratumor CD103(+) TILs was inversely associated with tumor size (p < 0.0001) and could represent a favorable prognostic predictor of overall and recurrence-free survival (p = 0.002 and 0.011, respectively). Moreover, intratumor CD103(+) TILs were positively associated with the expression of cognate ligand E-cadherin in intratumor regions of bladder urothelial cell carcinoma tissues (p = 0.008).

CONCLUSIONS:

Our findings suggest that CD8(+) T cells might have a significant role in tumor immunity by expressing CD103 in intratumor regions of bladder urothelial cell carcinoma tissues. Intratumor CD103(+) TILs could potentially serve as a prognostic marker in patients with bladder urothelial cell carcinoma.

• Authors: Bo Wang, Shaoxu Wu, Hong Zeng, Zhuowei Liu, Wen Dong, Wang He, Xu Chen, Xiaoliang Dong, Limin Zheng, Tianxin Lin, Jian Huang
• Journal: The Journal of urology (J Urol) Vol. 194 Issue 2 Pg. 556-62 (Aug 2015) ISSN: 1527-3792 [Electronic] United States
• PMID: 25752441 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

Chemical References

• Antigens, CD
• Biomarkers, Tumor
• Integrin alpha Chains
• alpha E integrins

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Antigens, CD (immunology, metabolism)
• Biomarkers, Tumor (analysis)
• Carcinoma, Transitional Cell (immunology, mortality, pathology)
• China (epidemiology)
• Disease-Free Survival
• Female
• Fluorescent Antibody Technique
• Humans
• Immunohistochemistry
• Integrin alpha Chains (immunology, metabolism)
• Kaplan-Meier Estimate
• Lymphocytes, Tumor-Infiltrating (immunology, metabolism, pathology)
• Male
• Middle Aged
• Neoplasm Staging
• Prognosis
• Proportional Hazards Models
• Retrospective Studies
• Survival Rate (trends)
• Urinary Bladder (immunology, metabolism, pathology)
• Urinary Bladder Neoplasms (immunology, mortality, pathology)
• Young Adult