Reduction of protein kinase C α (PKC-α) promote apoptosis via down-regulation of Dicer in bladder cancer.

Abstract

In clinic, we examined the expression of protein kinase C (PKC)-α and Dicer in the samples of bladder cancer patients, and found that the two proteins have a line correlation. Our study confirmed this correlation existing by clearing the decreasing expression of Dicer after the PKC-α knockdown. Treatment of bladder cancer cell lines (T24, 5637) with the PKC-α or Dicer knockdown and the PKC inhibitors (Calphostin C and Gö 6976) can promote the apoptosis. Inhibition of PKC can increase the activities of caspase-3 and PARP, however, decrease the expression of Dicer. And knockdown of the PKC-α or Dicer can also activate the caspase-3 or the PARP. Considering the reduction of PKC-α can induce the Dicer down-regulation, we make the conclusion that the reduction of PKC-α can promote the apoptosis via the down-regulation of Dicer in bladder cancer.

• Authors: Zhenming Jiang, Chuize Kong, Zhe Zhang, Yuyan Zhu, Yuxi Zhang, Xi Chen
• Journal: Journal of cellular and molecular medicine (J Cell Mol Med) Vol. 19 Issue 5 Pg. 1085-93 (May 2015) ISSN: 1582-4934 [Electronic] England
• PMID: 25752336 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

Chemical References

• Carbazoles
• Enzyme Inhibitors
• Naphthalenes
• Go 6976
• Poly(ADP-ribose) Polymerases
• PRKCA protein, human
• Protein Kinase C-alpha
• Ribonuclease III
• Caspase 3
• calphostin C

Topics

• Apoptosis (drug effects, genetics)
• Blotting, Western
• Carbazoles (pharmacology)
• Caspase 3 (metabolism)
• Cell Line, Tumor
• Down-Regulation
• Enzyme Inhibitors (pharmacology)
• Fluorescent Antibody Technique
• Humans
• Naphthalenes (pharmacology)
• Poly(ADP-ribose) Polymerases (metabolism)
• Protein Kinase C-alpha (antagonists & inhibitors, genetics, metabolism)
• RNA Interference
• Reverse Transcriptase Polymerase Chain Reaction
• Ribonuclease III (genetics, metabolism)
• Urinary Bladder Neoplasms (genetics, metabolism, pathology)