Abstract |
Diarrhea and pseudomembrane colitis caused by Clostridium difficile infection is a global health concern because of the high recurrence rate after standard antibiotic therapy. Vaccination presents a powerful countermeasure against disease recurrence. In this study, mice vaccinated with the nontoxigenic C. difficile membrane fraction generated a marked immune response to the antigen, as demonstrated by the serum IgG and intestinal fluid IgA levels. Significantly, pretreatment with harvested IgG- and IgA-containing fluids was sufficient to prevent in vitro adhesion of C. difficile to human Caco-2 intestinal cells. These results highlight the potential of nontoxigenic C. difficile membrane fraction as a vaccine candidate for C. difficile infection.
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Authors | Mitsutoshi Senoh, Masaaki Iwaki, Akihiko Yamamoto, Haru Kato, Tadashi Fukuda, Keigo Shibayama |
Journal | Microbial pathogenesis
(Microb Pathog)
Vol. 81
Pg. 1-5
(Apr 2015)
ISSN: 1096-1208 [Electronic] England |
PMID | 25745878
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Ltd. All rights reserved. |
Chemical References |
- Antibodies, Bacterial
- Bacterial Vaccines
- Immunoglobulin A
- Immunoglobulin G
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Topics |
- Animals
- Antibodies, Bacterial
(analysis, blood)
- Bacterial Adhesion
(drug effects)
- Bacterial Vaccines
(administration & dosage, immunology)
- Caco-2 Cells
- Clostridioides difficile
(immunology)
- Epithelial Cells
(microbiology)
- Humans
- Immunoglobulin A
(analysis)
- Immunoglobulin G
(blood)
- Intestinal Mucosa
(immunology)
- Mice
- Vaccination
(methods)
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