Abstract | BACKGROUND: The gut microbial ecosystem plays an important role in the pathogenesis of liver diseases. However, the association of microbial community structure with the severity of liver dysfunction is not completely understood. METHODS: RESULTS: Bifidobacterium (standardized regression coefficient [sβ] = -0.549; P < 0.001), Enterococcus (sβ = 0.369; P = 0.004), and yeast (sβ = 0.315; P = 0.018) numbers were independently associated with serum bilirubin, while Escherichia coli counts (sβ = 0.318; P = 0.046) correlated with INR, and Bifidobacterium counts (sβ = 0.410; P = 0.009) with serum creatinine. Only Bifidobacterium (sβ = -0.468; P = 0.003) and Enterococcus (sβ = 0.331; P = 0.029) counts were independent predictors of the MELD score. Bifidobacterium/Enterococcus ratio, proposed as a measure of pre-LT gut dysbiosis, was significantly related to the MELD score following the adjustment for the absolute Bifidobacterium (sβ = -0.333; P = 0.029) and Enterococcus (sβ = -0.966; P = 0.003) numbers. This pre-transplant dysbiosis ratio (PTDR) was significantly correlated with Enterococcus (R = -0.897; P < 0.001) but not with Bifidobacterium (R = 0.098; P = 0.546) counts. Among the other components of gut microflora, only hydrogen peroxide (H2 O2 )-producing Lactobacillus strains significantly influenced Enterococcus counts (sβ = 0.349; P = 0.028) and PTDR (sβ = -0.318; P = 0.046). CONCLUSION: While the abundance of both Bifidobacterium and Enterococcus is related to liver dysfunction, the size of the Enterococcus population seems to be the most important determinant of pre-LT gut dysbiosis in cirrhotic patients. The H2 O2 -producing Lactobacillus strains potentially ameliorate this dysbiotic state.
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Authors | M Grąt, W Hołówko, K M Wronka, K Grąt, Z Lewandowski, I Kosińska, M Krasnodębski, M Wasilewicz, M Gałęcka, P Szachta, H Zborowska, W Patkowski, M Krawczyk |
Journal | Transplant infectious disease : an official journal of the Transplantation Society
(Transpl Infect Dis)
Vol. 17
Issue 2
Pg. 174-84
(Apr 2015)
ISSN: 1399-3062 [Electronic] Denmark |
PMID | 25728703
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. |
Chemical References |
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Topics |
- Adult
- Aged
- Bifidobacterium
(isolation & purification)
- Bilirubin
(blood)
- Cohort Studies
- Creatinine
(blood)
- Dysbiosis
(blood, microbiology)
- End Stage Liver Disease
(blood, microbiology, surgery)
- Enterococcus
(isolation & purification)
- Escherichia coli
(isolation & purification)
- Feces
(microbiology)
- Female
- Gastrointestinal Microbiome
- Humans
- International Normalized Ratio
- Lactobacillus
(isolation & purification)
- Linear Models
- Liver Cirrhosis
(blood, microbiology, surgery)
- Liver Transplantation
- Male
- Middle Aged
- Prospective Studies
- Severity of Illness Index
- Yeasts
(isolation & purification)
- Young Adult
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