Co-induction by peroxisome proliferators of microsomal 1-acylglycerophosphocholine acyltransferase with peroxisomal beta-oxidation in rat liver.

Abstract

Administration of clofibric acid, 2,2'-(decamethylenedithio)diethanol, di(2-ethylhexyl)phthalate or perfluorooctanoic acid to male rates increased markedly microsomal 1-acylglycerophosphocholine (a-acyl-GPC) acyltransferase in a dose-dependent manner in liver. Simultaneous administration of actinomycin D or cycloheximide completely abolished the increase in the enzyme activity. The treatment of rats with clofibric acid did not affect the rate of decay of 1-acyl-GPC acyltransferase. Regardless of a great difference in the chemical structures of the peroxisome proliferators, high correlation was observed between the induced activities of microsomal 1-acyl-GPC acyltransferase and peroxisomal beta-oxidation. Stearoyl-CoA desaturase was induced by peroxisome proliferators in a dose-dependent manner; nevertheless, high correlation was not seen between the induced activities of desaturase and peroxisomal beta-oxidation. Hormonal (adrenalectomy, diabetes, hyperthyroidism and hypothyroidism) and nutritional (starvation, starvation-refeeding, fat-free diet feeding and high-fat diet feeding) alterations hardly affected the activity of 1-acyl-GPC acyltransferase. The present results indicate that microsomal 1-acyl-GPC acyltransferase is a useful parameter responsive to the challenges by peroxisome proliferators and suggest that a similar regulatory mechanism operates for the inductions of microsomal 1-acyl-GPC acyltransferase and peroxisomal beta-oxidation.

• Authors: Y Kawashima, S Horii, T Matsunaga, A Hirose, T Adachi, H Kozuka
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1005 Issue 2 Pg. 123-9 (Sep 25 1989) ISSN: 0006-3002 [Print] Netherlands
• PMID: 2570610 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hypolipidemic Agents
• Stearoyl-CoA Desaturase
• Acyltransferases
• 1-Acylglycerophosphocholine O-Acyltransferase

Topics

• 1-Acylglycerophosphocholine O-Acyltransferase
• Acyltransferases (biosynthesis)
• Animals
• Enzyme Induction (drug effects)
• Hypolipidemic Agents (pharmacology)
• In Vitro Techniques
• Male
• Microbodies (drug effects, enzymology)
• Microsomes, Liver (drug effects, enzymology)
• Oxidation-Reduction
• Rats
• Rats, Inbred Strains
• Stearoyl-CoA Desaturase (metabolism)