Amelioration of ischemia-reperfusion-induced muscle injury by the recombinant human MG53 protein.

Abstract

INTRODUCTION:

Ischemia-reperfusion injury (I-R) in skeletal muscle requires timely treatment.

METHODS:

Rodent models of I-R injury were used to test the efficacy of recombinant human MG53 (rhMG53) protein for protecting skeletal muscle.

RESULTS:

In a mouse I-R injury model, we found that mg53,-/- mice are more susceptible to I-R injury. rhMG53 applied intravenously to the wild-type mice protected I-R injured muscle, as demonstrated by reduced CK release and Evans blue staining. Histochemical studies confirmed beneficial effects of rhMG53. Of interest, rhMG53 did not protect against I-R injury in rat skeletal muscle. This was likely due to the fact that the plasma level of endogenous MG53 protein is high in rats.

CONCLUSIONS:

Our data suggest that rhMG53 may be a potential therapy for protection against muscle trauma. A mouse model appears to be a better choice than a rat model for evaluating potential treatments for protecting skeletal muscle.

• Authors: Hua Zhu, Jincai Hou, Janet L Roe, Ki Ho Park, Tao Tan, Yongqiu Zheng, Lei Li, Cuixiang Zhang, Jianxun Liu, Zhenguo Liu, Jianjie Ma, Thomas J Walters
• Journal: Muscle & nerve (Muscle Nerve) Vol. 52 Issue 5 Pg. 852-8 (Nov 2015) ISSN: 1097-4598 [Electronic] United States
• PMID: 25703692 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
• Copyright: © 2015 Wiley Periodicals, Inc.

Chemical References

• Carrier Proteins
• Recombinant Proteins
• TRIM72 protein, human
• Tripartite Motif Proteins

Topics

• Animals
• Carrier Proteins (pharmacology, therapeutic use)
• Humans
• Male
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Muscle, Skeletal (drug effects, injuries, pathology)
• Rats
• Rats, Sprague-Dawley
• Recombinant Proteins (pharmacology, therapeutic use)
• Reperfusion Injury (blood, drug therapy, pathology)
• Tripartite Motif Proteins