Negative HER2/neu amplification using immunohistochemistry and chromogenic in situ hybridization techniques in skin melanoma cases.

Abstract

BACKGROUND:

This study was performed to evaluate the amplification of HER-2/neu in patients with melanoma.

MATERIALS AND METHODS:

Amplification of HER-2/neu was evaluated in a group of patients with melanoma, referred to two referral centers in Tehran, using immunohistochemistry (IHC) and chromogenic in situ hybridization (CISH) techniques.

RESULTS:

Forty patients with mean age 57.9±19.5 years were enrolled in this study. The most frequent type of melanoma was acral, while lower limbs were the most frequent sites. The amplification of HER2/ neu was negative in 97.5% of patients with IHC and in 100% of patients with CISH technique. Only one case (2.5%) shows weak positive staining (+2) in IHC method. Fifty five percent of melanoma was ulcerative, and the most common stages of tumors were stages 4b and 3b. More than 47% of cases were in Clark level III, while the mean of Breslow thickness was 3.56±2.87 mm. The stage of the case that showed weakly positive staining (2+) in IHC was 4b.

CONCLUSIONS:

The amplification of HER2/neu biomarker was negative in patients with melanoma, using both CISH and IHC techniques.

• Authors: Nasrin Shayanfar, Leila Bahari, Zahra Safaie-Naraghi, Kambiz Kamyab, Elmira Gheytanchi, Nima Rezaei
• Journal: Asian Pacific journal of cancer prevention : APJCP (Asian Pac J Cancer Prev) Vol. 16 Issue 2 Pg. 421-5 ( 2015) ISSN: 2476-762X [Electronic] Thailand
• PMID: 25684465 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• ERBB2 protein, human
• Receptor, ErbB-2

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Biomarkers, Tumor (genetics, metabolism)
• Child
• Cross-Sectional Studies
• Female
• Follow-Up Studies
• Gene Amplification
• Humans
• Immunoenzyme Techniques
• In Situ Hybridization
• Male
• Melanoma (genetics, metabolism, pathology)
• Middle Aged
• Neoplasm Staging
• Prognosis
• Receptor, ErbB-2 (genetics, metabolism)
• Skin Neoplasms (genetics, metabolism, pathology)
• Young Adult