MiR-210 links hypoxia with cell proliferation regulation in human Laryngocarcinoma cancer.

Abstract

The microRNA hsa-miR-210 (miR-210) is associated with hypoxia; however its function has not fully identified. In the present study, we aim to detect its role concerning proliferation in Laryngocarcinoma. We found that miR-210 was highly expressed in hypoxia, which inhibited proliferation by inducing cell cycle arrest in G1/G0 as well as apoptosis. We further identified that miR-210 targeted fibroblast growth factor receptor-like 1 (FGFRL1). Down regulation of FGFRL1 decreased cell proliferation by promoting proportion of cells in G1/G0 phase and decreasing in S and G2/M phases. Moreover, overexpression of FGFRL1 effectively released the miR-210-induced suppression of SCC10A cell proliferation. Expression of miR-210 repressed tumor xenograft growth in vivo as well. Together, our findings reveal a new mechanism of adaptation to hypoxia that miR-210 inhibits the proliferation via inducing cell cycle arrest and apoptosis by the targeting of FGFRL1. J. Cell. Biochem. 116: 1039-1049, 2015. © 2015 Wiley Periodicals, Inc.

• Authors: Jianhong Zuo, Meiling Wen, Mingsheng Lei, Xiang Peng, Xuefeng Yang, Zhigang Liu
• Journal: Journal of cellular biochemistry (J Cell Biochem) Vol. 116 Issue 6 Pg. 1039-49 (Jun 2015) ISSN: 1097-4644 [Electronic] United States
• PMID: 25639884 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 Wiley Periodicals, Inc.

Chemical References

• MIRN210 microRNA, human
• MicroRNAs

Topics

• Animals
• Blotting, Western
• Cell Cycle
• Cell Line, Tumor
• Cell Proliferation (genetics, physiology)
• Gene Expression Regulation, Neoplastic
• Head and Neck Neoplasms (genetics, metabolism)
• Humans
• Immunohistochemistry
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• MicroRNAs (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction