Evaluation of vector control is crucial for improving
malaria containment and, according to World Health Organization, new complementary indicators would be very valuable. In this study the
IgG response to the Anopheles-specific cE5
salivary protein was tested as a tool to evaluate the efficacy of
insecticide-treated nets in reducing human exposure to
malaria vectors. Sera collected during a longitudinal study carried out in Angola, and including entomological and parasitological data, were used to assess the
IgG response to the Anopheles gambiae cE5 in both children and adults, before and after the application of
insecticide-treated nets. Seasonal fluctuation of specific
IgG antibody levels according to exposure was only found in children (up to ≈ 14 years old) whose anti-cE5
IgG response dropped after bed nets installation. These results were fully consistent with previous findings obtained with the same set of sera and indicating a substantial reduction of human-vector contact shortly after nets implementation. Overall, children
IgG response to the cE5
protein appeared a very sensitive
biomarker, which allowed for the detection of even weak exposure to Anopheles
bites, indicating it may represent a reliable additional tool to evaluate the efficacy of vector control interventions.