The effective management of
tumors resistant to
platinum drugs-based anticancer
therapies is a critical challenge in current clinical practices. The proapoptotic Bcl-2 family
proteins Bax and Bak are essential for
cisplatin-induced apoptosis. Unfortunately, Bax and its related upstream endogenous apoptotic signaling pathways are often dysregulated in
cancer cells. Strategies that are able to bypass Bax- and Bak-dependent apoptotic pathways will thus provide opportunities to overcome
platinum drug resistance. We have identified the thioxodihydroquinazolinone
mdivi-1 as a member of a novel class of small molecules that are able to induce Bax- and Bak-independent mitochondrial outer membrane permeabilization when combined with
cisplatin, thereby efficiently triggering apoptosis in
platinum-resistant
tumor cells. In the present structure activity relationship (SAR) study of a computationally selected library of
mdivi-1 related small molecules, we established a pharmacophore model that can lead to the enhancement of
platinum drug efficacy and Bax/Bak-independent mitochondrial apoptosis. Specifically, we found that a
thiourea function is necessary but not sufficient for the synergism of this class of thioxodihydroquinazolinones with
cisplatin. We were also able to identify more potent
mdivi-1 analogs through this SAR study, which will guide future designs with the goal to develop novel combination regimens for the treatment of
platinum- and multidrug-resistant
tumors.