Abstract |
Minimally invasive therapies are increasingly in demand for organ-confined prostate tumors. Electrochemical therapy (EChT) is attractive, as it relies on locally-induced reduction-oxidation reactions to kill tumor cells. Its efficacy for prostate cancer was assessed in human PC-3 and LNCaP tumor xenografts growing subcutaneously in nude mice (n = 80) by applying 2 Stainless Steel vs. 4 Platinum- Iridium (Pt-Ir) electrodes to deliver current densities of 10 to 35 mA/cm(2) for 30 or 60 min. The procedure was uneventful in 90% of mice. No difference in tumor vs. body temperature was observed. Changes at electrode- tumor junctions were immediate, with dryness and acidity (pH2-3) at the anode and oedema and alkalinity (pH10-12) at the cathode. This was accompanied by cellular alterations, found more pronounced at the cathode. Such acidic and alkaline conditions were cytotoxic in vitro and dissolved cells at pH>10. In mice, tumor destruction was extensive by 24h with almost undetectable blood prostate specific antigen (LNCaP model) and covered the whole tumor surface by 4 days. EChT was most efficient at 25-30 mA/cm(2) for 60 min, yielding the longest recurrence-free survival and higher cure rates, especially with 4 Pt-Ir electrodes. EChT is a promising option to optimize for organ-confined prostate tumors.
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Authors | Fabio L Cury, Bimal Bhindi, Joice Rocha, Eleonora Scarlata, Katia El Jurdi, Michel Ladouceur, Stéphane Beauregard, Ashok K Vijh, Yosh Taguchi, Simone Chevalier |
Journal | Bioelectrochemistry (Amsterdam, Netherlands)
(Bioelectrochemistry)
Vol. 104
Pg. 1-9
(Aug 2015)
ISSN: 1878-562X [Electronic] Netherlands |
PMID | 25578541
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier B.V. All rights reserved. |
Topics |
- Animals
- Cell Line, Tumor
- Cell Transformation, Neoplastic
- Electric Stimulation Therapy
(methods)
- Electrochemistry
- Feasibility Studies
- Humans
- Male
- Mice
- Mice, Nude
- Oxidation-Reduction
- Prostatic Neoplasms
(metabolism, pathology, therapy)
- Time Factors
- Treatment Outcome
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