Direct ESR measurement of free radicals in mouse pancreatic lesions.
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| Abstract |
In this experiment, free radicals in the pancreas of endotoxemia and ethionine induced acute pancreatitis in mice were attempted to be detected directly by ESR spectroscopy, using 77 K freeze-trapping and 25 degrees C DMPO spin trapping techniques. In the 77 K freeze-trapping method, Mn (II) ion and R-00. radical were detected in endotoxemia and ethionine induced pancreatic lesions. The heme-NO radical was observed at 6 and 24 h after isolation of the normal pancreas, and signal intensity was increased with time. This finding supports that ESR spectroscopy is a useful method for detecting the tissue degeneration process from ischemia to necrosis. Using the DMPO spin trapping technique (25 degrees C), 6-line was detected at 6 h after intraperitoneal administration of E. coli in the model of endotoxemia, and 3- and 6-lines and a signal suggestive of DMPO-OH adduct were noted at 12 and 24 h in ethionine pancreatitis. These findings suggest that impaired pancreatic tissues exist in a considerably oxidative environment and oxygen derived free radicals may be considered to play an important role in the development of pancreatic lesions.
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| Authors | A Nonaka, T Manabe, N Asano, T Kyogoku, K Imanishi, K Tamura, T Tobe, Y Sugiura, K Makino |
| Journal | International journal of pancreatology : official journal of the International Association of Pancreatology (Int J Pancreatol) Vol. 5 Issue 2 Pg. 203-11 (Sep 1989) ISSN: 0169-4197 [Print] United States |
| PMID | 2557371 (Publication Type: Journal Article) |
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